Hayakawa T, Zhu Y, Itoh K, Kimura Y, Izawa T, Shimamoto K, Toriyama S
Plantech Research Institute, Yokohama, Japan.
Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9865-9. doi: 10.1073/pnas.89.20.9865.
The coat protein (CP) gene of rice stripe virus was introduced into two japonica varieties of rice by electroporation of protoplasts. The resultant transgenic plants expressed the CP at high levels (up to 0.5% of total soluble protein) and exhibited a significant level of resistance to virus infection. Plants derived from selfed progeny of the primary transformants also expressed the CP and showed viral resistance, indicating stable transmission of the CP gene and the trait of resistance to the next generation. Moreover, the virally encoded strip disease-specific protein was not detected in transgenic plants expressing CP 8 weeks after inoculation, indicating protection before viral multiplication. These studies demonstrated that CP-mediated resistance to virus infection can be extended to cereals and to the viruses transmitted by an insect vector (planthopper).
通过原生质体电穿孔法将水稻条纹病毒的外壳蛋白（CP）基因导入两个粳稻品种。所得转基因植物高水平表达CP（高达总可溶性蛋白的0.5%），并表现出对病毒感染的显著抗性。来自初级转化体自交后代的植株也表达CP并表现出病毒抗性，表明CP基因和抗性性状能稳定遗传至下一代。此外，接种8周后，在表达CP的转基因植物中未检测到病毒编码的条纹病特异性蛋白，表明在病毒增殖前就受到了保护。这些研究表明，CP介导的对病毒感染的抗性可扩展到谷类作物以及由昆虫介体（飞虱）传播的病毒。
