Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Roque Saenz Pena 352, Bernal, B1876BXD, Buenos Aires, Argentina.
Med Chem. 2012 Mar;8(2):222-9. doi: 10.2174/157340612800493584.
Arsenic compounds have shown medical usefulness since they proved to be effective in causing complete remission of acute promyelocytic leukemia. In this work we obtained a fluorescently labeled arsenic compound that can be used with current fluorescence techniques for basic and applied research, focused on arsenic-induced apoptosis studies. This compound is an arsanilic acid bearing a covalently linked FITC that was chemically synthesized and characterized by fluorescence, UV-Vis, mass and FTIR spectrometry. In addition, we assessed its apoptotic activity as well as its fluorescent labeling properties in HL60 cell line as a leukemia cell model through flow cytometry. We obtained a compound with a 1:1 FITC:arsenic ratio and a 595 m/z, confirming its structure by FTIR. This compound proved to be useful at inducing apoptosis in the leukemia cell model and labeling this apoptotic cell population, in such a way that the highest FITC fluorescence correlated with the highest arsenic amount.
砷化合物在证明对急性早幼粒细胞白血病有完全缓解作用后，显示出了医学用途。在这项工作中，我们获得了一种荧光标记的砷化合物，可与当前的荧光技术一起用于基础和应用研究，重点是砷诱导细胞凋亡的研究。这种化合物是一种带有共价连接的 FITC 的对氨基苯砷酸，通过荧光、UV-Vis、质谱和傅里叶变换红外光谱法进行了化学合成和表征。此外，我们通过流式细胞术评估了其在 HL60 细胞系（白血病细胞模型）中的促凋亡活性和荧光标记特性。我们获得了一种 FITC:砷摩尔比为 1:1，分子量为 595 m/z 的化合物，并通过傅里叶变换红外光谱法证实了其结构。该化合物在诱导白血病细胞模型细胞凋亡和标记该凋亡细胞群体方面非常有效，最高的 FITC 荧光与最高的砷含量相关。
