Tsvetkova T A, Gorokhova T A, Del'vig A A
Vopr Med Khim. 1990 Jul-Aug;36(4):53-5.
Relative synthesis of collagen was studied in skin fibroblasts of children with funnel chest and of corresponding age children (control) in presence of ascorbic acid. In presence of ascorbic acid the rate of collagen synthesis was 2-2.4-fold lower that in corresponding controls both in proliferating and stationary cultures. At the same time, relative synthesis of collagen was quite similar both in the patient and control fibroblasts in presence of ascorbic acid. Estimation of the 14C-Hypro/14C-Pro ratio in collagens isolated from cultural media showed that there was no difference in the hydroxylation rate of collagens in control and patient fibroblasts. These data suggest that funnel chest is one of the forms of systemic connective tissue diseases, which impaired mainly the cartilages.
在抗坏血酸存在的情况下，研究了漏斗胸患儿及相应年龄对照组儿童皮肤成纤维细胞中胶原蛋白的相对合成情况。在抗坏血酸存在时，无论是增殖培养还是静止培养，胶原蛋白的合成速率均比相应对照组低2至2.4倍。同时，在抗坏血酸存在时，患者和对照组成纤维细胞中胶原蛋白的相对合成情况相当相似。对从培养基中分离出的胶原蛋白中14C-羟脯氨酸/14C-脯氨酸比率的评估表明，对照和成纤维细胞中胶原蛋白的羟基化速率没有差异。这些数据表明，漏斗胸是系统性结缔组织疾病的一种形式，主要损害软骨。
