National Center for Genetic Engineering and Biotechnology, National Sciences and Technology Development Agency at King Mongkut's University of Technology Thonburi (Bangkhuntien), Bangkok, Thailand.
Biosens Bioelectron. 2012 Apr 15;34(1):238-43. doi: 10.1016/j.bios.2012.02.011. Epub 2012 Feb 14.
Bioconjugate nanocapsules were fabricated by using polystyrene sulfonate (PSS) to encapsulate gold nanoparticles (AuNPs) bearing adsorbed horseradish peroxidase (HRP). The average size of nanocapsule was in a range 150-400 nm. The efficiency of the capsules to enhance signals in an immunoassay was demonstrated by using an enzyme linked immunosorbent assay (ELISA) to detect the food-borne pathogen -Listeria monocytogenes. The antibody adsorbed onto the PSS shell of the nanocapsules provided the recognition molecule. For a given quantity of antibody, the bioconjugate nanocapsules showed 30 times greater sensitivity and a shorter assay time (5 min) when compared to conventional ELISA using an HRP labelled antibody. This proof-of-concept encapsulation of HRP through PSS nanocapsules may pave the way for alternative signal enhancement strategies where sensitivity is a priority.
生物共轭纳米胶囊是通过使用聚苯乙烯磺酸盐（PSS）来包裹带有吸附辣根过氧化物酶（HRP）的金纳米颗粒（AuNPs）制成的。纳米胶囊的平均尺寸在 150-400nm 范围内。通过使用酶联免疫吸附测定（ELISA）检测食源性病原体-单核细胞增生李斯特菌，证明了纳米胶囊在免疫测定中增强信号的效率。吸附在纳米胶囊 PSS 壳上的抗体提供了识别分子。对于给定数量的抗体，与使用 HRP 标记抗体的传统 ELISA 相比，生物共轭纳米胶囊显示出 30 倍的灵敏度和更短的测定时间（5 分钟）。通过 PSS 纳米胶囊对 HRP 的这种概念验证封装可能为替代信号增强策略铺平道路，在这些策略中，灵敏度是优先考虑的。
