Lin M H, Romsos D R, Akera T, Leveille G A
Experientia. 1979 Jun 15;35(6):735-6. doi: 10.1007/BF01968211.
(3H)-Ouabain binding to liver and kidney preparations was utilized to estimate the number of Na+, K+-ATPase enzyme units in livers and kidneys from rats fed 2% corn oil supplemented or fat-free diets. The specific (3H)-ouabain binding in liver and kidney preparations from fatty acid deficient rats was increased approximately 40%, but the affinity of the binding sites for ouabain (Kd-value) remained unchanged. The increased concentration of Na+, K+-ATPase enzyme units observed in the essential fatty acid deficient rats may contribute to the reduced body fat accumulation and elevated heat production observed in these animals.
利用（3H）哇巴因与肝脏和肾脏制剂的结合来估计喂食2%玉米油补充饲料或无脂肪饲料的大鼠肝脏和肾脏中Na +，K + -ATP酶单位的数量。脂肪酸缺乏大鼠的肝脏和肾脏制剂中特异性（3H）哇巴因结合增加了约40%，但结合位点对哇巴因的亲和力（Kd值）保持不变。在必需脂肪酸缺乏的大鼠中观察到的Na +，K + -ATP酶单位浓度增加可能有助于这些动物体内脂肪积累减少和产热增加。
