• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

北非黑曼巴蛇和黑颈眼镜蛇种群的毒液组学和抗毒液组学特征揭示了一个潜在的重要治疗弱点。

Venomics and antivenomics profiles of North African Cerastes cerastes and C. vipera populations reveals a potentially important therapeutic weakness.

机构信息

Venoms and Toxins Laboratory, Pasteur Institute of Morocco, 1. Place Louis Pasteur, 20100-Casablanca, Morocco.

出版信息

J Proteomics. 2012 Apr 18;75(8):2442-53. doi: 10.1016/j.jprot.2012.02.021. Epub 2012 Feb 24.

DOI:10.1016/j.jprot.2012.02.021
PMID:22387316
Abstract

We report the proteomic analysis of the venom of the medically relevant snake, Cerastes cerastes, from Morocco, and the immunoreactivity profile of an experimental monospecific (CcMo_AV against Moroccan C. cerastes venom) and a commercial (Gamma-VIP against Tunisian C. cerastes and M. lebetina venoms) F(ab')(2) antivenoms towards geographic variants of C. cerastes and C. vipera venoms. The venom of C. cerastes is a low-complexity proteome composed of 25-30 toxins belonging to 6 protein families, mainly targetting the hemostatic system. This toxin arsenal explains the clinical picture observed in C. cerastes envenomings. Despite geographic compositional variation, the monospecific CcMo_AV and the Gamma-VIP divalent antivenom produced at Institut Pasteur de Tunis, showed similar immunocapturing capability towards Moroccan, Tunisian, and Egyptian C. cerastes venom proteins. Proteins partially escaping immunorecognition were all identified as PLA(2) molecules. Antivenomic analysis showed low degree of cross-reactivity of Moroccan CcMo_AV and Tunisian Gamma-VIP antivenoms towards C. vipera venom toxins. This study indicates that a more complete therapeutic cover could be achieved by including C. vipera venom in the formulation of venom immunization mixtures, thereby generating a pan-Cerastes antivenom.

摘要

我们报告了来自摩洛哥具有医学相关性的蛇 Cerastes cerastes 的毒液的蛋白质组学分析，以及一种实验性单特异性（CcMo_AV 针对摩洛哥 C. cerastes 毒液）和一种商业性（Gamma-VIP 针对突尼斯 C. cerastes 和 M. lebetina 毒液） F(ab')(2) 抗蛇毒血清对 C. cerastes 和 C. vipera 毒液地理变异体的免疫反应谱。Cerastes cerastes 的毒液是一种低复杂度的蛋白质组，由 25-30 种毒素组成，属于 6 种蛋白质家族，主要针对止血系统。这种毒素库解释了在 C. cerastes 中毒时观察到的临床症状。尽管存在地理成分的变化，但在巴斯德研究所生产的单特异性 CcMo_AV 和 Gamma-VIP 二价抗蛇毒血清对摩洛哥、突尼斯和埃及的 C. cerastes 毒液蛋白表现出相似的免疫捕获能力。部分逃避免疫识别的蛋白均被鉴定为 PLA(2)分子。抗蛇毒组学分析表明，摩洛哥的 CcMo_AV 和突尼斯的 Gamma-VIP 抗蛇毒血清对 C. vipera 毒液毒素的交叉反应程度较低。这项研究表明，通过在毒液免疫混合物的配方中加入 C. vipera 毒液，可以实现更全面的治疗覆盖，从而产生一种泛 Cerastes 抗蛇毒血清。

相似文献

1
Venomics and antivenomics profiles of North African Cerastes cerastes and C. vipera populations reveals a potentially important therapeutic weakness.北非黑曼巴蛇和黑颈眼镜蛇种群的毒液组学和抗毒液组学特征揭示了一个潜在的重要治疗弱点。
J Proteomics. 2012 Apr 18;75(8):2442-53. doi: 10.1016/j.jprot.2012.02.021. Epub 2012 Feb 24.
2
Snake venomics of Macrovipera mauritanica from Morocco, and assessment of the para-specific immunoreactivity of an experimental monospecific and a commercial antivenoms.来自摩洛哥的棕伊澳蛇的蛇毒组学，以及实验性单特异性和商业抗蛇毒血清的类特异性免疫反应评估。
J Proteomics. 2012 Apr 18;75(8):2431-41. doi: 10.1016/j.jprot.2012.02.022. Epub 2012 Feb 24.
3
Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina.蛇类毒液组学：突尼斯蛇类角蝰、锯鳞蝰和北非锯鳞蝰毒液蛋白质组的比较分析
Proteomics. 2005 Nov;5(16):4223-35. doi: 10.1002/pmic.200402024.
4
Venomics and antivenomics of Bothrops erythromelas from five geographic populations within the Caatinga ecoregion of northeastern Brazil.巴西东北部卡廷加生态区域内五个地理种群的红吻蝰蛇的蛇毒组学和抗蛇毒组学研究
J Proteomics. 2015 Jan 30;114:93-114. doi: 10.1016/j.jprot.2014.11.011. Epub 2014 Nov 21.
5
Bioinformatics and multiepitope DNA immunization to design rational snake antivenom.生物信息学与多表位DNA免疫法用于设计合理的蛇毒抗血清。
PLoS Med. 2006 Jun;3(6):e184. doi: 10.1371/journal.pmed.0030184.
6
Snake venomics and antivenomics of the arboreal neotropical pitvipers Bothriechis lateralis and Bothriechis schlegelii.树栖新热带蝰蛇侧带棕榈蝰和施氏棕榈蝰的蛇毒组学与抗蛇毒组学
J Proteome Res. 2008 Jun;7(6):2445-57. doi: 10.1021/pr8000139. Epub 2008 Apr 30.
7
Extreme Procoagulant Potency in Human Plasma of Venoms from the African Viperid Genera and and the Relative Efficacy of Antivenoms and Synthetic Enzyme-Inhibitors.人类血浆中来自非洲蝰蛇属 和 的极强烈促凝血效力，以及抗蛇毒血清和合成酶抑制剂的相对疗效。
Toxins (Basel). 2022 Dec 1;14(12):836. doi: 10.3390/toxins14120836.
8
Snake venomics and antivenomics of Crotalus durissus subspecies from Brazil: assessment of geographic variation and its implication on snakebite management.巴西响尾蛇亚种的蛇毒学和抗蛇毒血清学研究：地理变异评估及其对蛇伤管理的影响。
J Proteomics. 2010 Aug 5;73(9):1758-76. doi: 10.1016/j.jprot.2010.06.001. Epub 2010 Jun 11.
9
Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management.矛头蝮属蛇类的种群毒液学和抗蛇毒血清学研究：在其穿越亚马逊的扩散过程中的幼态持续现象，以及地理毒液变异对蛇伤管理的影响。
J Proteomics. 2011 Apr 1;74(4):510-27. doi: 10.1016/j.jprot.2011.01.003. Epub 2011 Jan 26.
10
Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism.来自哥伦比亚、巴西亚马逊地区、秘鲁和厄瓜多尔的矛头蝮属蛇毒的蛇毒学和抗蛇毒血清组学研究表明，毒液表型存在地理变异，这种变异倾向于幼态持续现象。
J Proteomics. 2009 Nov 2;73(1):57-78. doi: 10.1016/j.jprot.2009.07.013. Epub 2009 Aug 6.

引用本文的文献

1
Design, development and preclinical assessment of MENAVip-ICP, a new snake antivenom with potential coverage of species in the Middle East and North Africa regions.MENAVip-ICP的设计、开发及临床前评估，一种有望覆盖中东和北非地区蛇类的新型抗蛇毒血清。
Toxicon X. 2024 Aug 30;24:100206. doi: 10.1016/j.toxcx.2024.100206. eCollection 2024 Dec.
2
Structural, Biochemical Characterization and Molecular Mechanism of Cerastokunin: A New Kunitz-Type Peptide with Potential Inhibition of Thrombin, Factor Xa and Platelets.塞拉托昆丁的结构、生化特性及分子机制研究：一种新型的 Kunitz 型肽，具有潜在的抗凝血酶、因子 Xa 和血小板的作用。
Protein J. 2024 Aug;43(4):888-909. doi: 10.1007/s10930-024-10226-9. Epub 2024 Aug 2.
3
Insight into the Toxicological and Pathophysiological Effects of Moroccan Vipers' Venom: Assessing the Efficacy of Commercial Antivenom for Neutralization.
深入了解摩洛哥蝰蛇毒液的毒理学和病理生理学效应：评估商业抗蛇毒血清的中和效力。
Trop Med Infect Dis. 2023 May 31;8(6):302. doi: 10.3390/tropicalmed8060302.
4
High-throughput proteomics and in vitro functional characterization of the 26 medically most important elapids and vipers from sub-Saharan Africa.对撒哈拉以南非洲地区 26 种医学上最重要的眼镜蛇和蝰蛇进行高通量蛋白质组学和体外功能特征分析。
Gigascience. 2022 Dec 13;11. doi: 10.1093/gigascience/giac121.
5
A Review of the Proteomic Profiling of African Viperidae and Elapidae Snake Venoms and Their Antivenom Neutralisation.非洲蝰科和眼镜蛇科蛇毒的蛋白质组学分析及其抗蛇毒血清中和作用的综述
Toxins (Basel). 2022 Oct 22;14(11):723. doi: 10.3390/toxins14110723.
6
Bibliometric Analysis of Literature in Snake Venom-Related Research Worldwide (1933-2022).全球蛇毒相关研究文献的文献计量分析（1933 - 2022）
Animals (Basel). 2022 Aug 12;12(16):2058. doi: 10.3390/ani12162058.
7
Therapeutic Outcome of Anti-inflammatory and Antioxidative Medicines on the Dermonecrotic Activity of Cerastes cerastes Venom.抗炎和抗氧化药物对角蝰蛇毒皮肤坏死活性的治疗效果。
Inflammation. 2022 Aug;45(4):1700-1719. doi: 10.1007/s10753-022-01654-9. Epub 2022 Mar 5.
8
Terrestrial venomous animals, the envenomings they cause, and treatment perspectives in the Middle East and North Africa.中东和北非地区的陆生有毒动物、由其导致的中毒事件及治疗前景。
PLoS Negl Trop Dis. 2021 Dec 2;15(12):e0009880. doi: 10.1371/journal.pntd.0009880. eCollection 2021 Dec.
9
Old World Vipers-A Review about Snake Venom Proteomics of Viperinae and Their Variations.旧世界蝰蛇——关于蝰蛇科蛇毒蛋白质组学及其变异的综述。
Toxins (Basel). 2021 Jun 17;13(6):427. doi: 10.3390/toxins13060427.
10
Isolation and Functional Identification of an Antiplatelet RGD-Containing Disintegrin from Cerastes cerastes Venom.从沙漠蝰蛇毒液中分离并鉴定一种抗血小板含精氨酸-甘氨酸-天冬氨酸（RGD）的解整合素。
Protein J. 2020 Oct;39(5):574-590. doi: 10.1007/s10930-020-09915-y. Epub 2020 Sep 22.