Department of Chemistry, School of Science, Wuhan University of Technology, Wuhan, PR China.
Environ Toxicol Pharmacol. 2012 May;33(3):377-85. doi: 10.1016/j.etap.2012.01.009. Epub 2012 Feb 6.
The toxicity of chlorobenzenes to Tetrahymena growth metabolism was studied by microcalorimetry. The growth constant (k), peak time (T) and generation times (T(G)) were calculated. IC(50) of chlorobenzenes was obtained through the kinetic parameters. The results suggested that the order of toxicity was 1,2,4-trichlorobenzene>o-dichlorobenzene>p-dichlorobenzene>m-dichlorobenzene>chlorobenzene. ATR-FTIR spectra revealed that amide groups and PO(2)(-) of the phospholipid phospho-diester, both in the hydrophobic end exposed to the outer layer, were the easiest to be damaged. The relationship between IC(50) and chemicals structure parameters (E(LUMO), E(HOMO), logK(OW), ∑Q(R), ΔQ(πR) and ΔE), indicated that the more chlorine atoms were substituted, the greater the toxicity was. Chlorobenzenes have toxicity of non-polar narcosis. Their toxicity is proportional to their concentrations at the site of action, and caused by membrane perturbation.
采用微量热法研究了氯苯对四膜虫生长代谢的毒性。计算了生长常数（k）、峰时（T）和世代时间（T(G)）。通过动力学参数得到了氯苯的 IC(50)。结果表明，毒性顺序为 1,2,4-三氯苯>邻二氯苯>对二氯苯>间二氯苯>氯苯。ATR-FTIR 光谱表明，磷脂磷酸二酯的酰胺基团和 PO(2)(-)，都在疏水区暴露在外部，最容易被破坏。IC(50)与化学结构参数（E(LUMO)、E(HOMO)、logK(OW)、∑Q(R)、ΔQ(πR)和ΔE）之间的关系表明，取代的氯原子越多，毒性越大。氯苯具有非极性麻醉毒性。它们的毒性与其在作用部位的浓度成正比，是由膜扰动引起的。
