Centro de Investigación en Ciencia y Tecnología de los Alimentos, Instituto de Ciencias Agropecuarias, UAEH, Av. Universitaria Km. 1. Exhac. de Aquetzalpa, 43600, Tulancingo, Hidalgo, México.
Folia Microbiol (Praha). 2012 Mar;57(2):149-58. doi: 10.1007/s12223-012-0106-9. Epub 2012 Mar 3.
Pleurotus ostreatus degrades polychlorinated biphenyls (PCBs) with an increase of laccase activity. Laccases are well known for their detoxifying activity. We show, using reverse transcription polymerase chain reaction and a biochemical assay, that reduction in PCBs (di, tri, tetra, and penta) levels are correlated with an increase in laccase activity. P. ostreatus cultures were obtained from 0 to 30 days in the presence or absence of 7,100 mg/L PCBs (from transformer oil) and a surfactant. After each selected time cultures were withdrawn and remaining PCBs were determined, a maximal removal percentage of PCBs was obtained at 20 (63.5 ± 2.0) and 30 days (63.8 ± 4.6) post-induction. Also, the activity of the enzyme was analyzed and it was found to increase at 10 (6.9-fold) and 20 (6.77-fold) days post-induction in the presence of PCBs, as determined by its activity. Taken together, these data suggest that PCBs induce laccase expression and that laccase catalyzes PCBs removal.
糙皮侧耳（Pleurotus ostreatus）可通过增加漆酶活性来降解多氯联苯（PCBs）。漆酶以其解毒活性而闻名。我们通过反转录聚合酶链反应和生化分析表明，PCBs（二氯、三氯、四氯和五氯）水平的降低与漆酶活性的增加相关。在存在或不存在 7,100 mg/L 多氯联苯（来自变压器油）和表面活性剂的情况下，从 0 天到 30 天获得糙皮侧耳培养物。在每个选定的时间点取出培养物并确定剩余的 PCBs 后，在诱导后 20 天（63.5±2.0）和 30 天（63.8±4.6）时获得了最大的 PCB 去除百分比。此外，通过其活性分析发现，在存在 PCBs 的情况下，酶的活性在诱导后 10 天（6.9 倍）和 20 天（6.77 倍）时增加。综上所述，这些数据表明 PCBs 诱导漆酶表达，而漆酶催化 PCBs 的去除。
