Department of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
J Pept Sci. 2012 Apr;18(4):224-32. doi: 10.1002/psc.1437. Epub 2012 Mar 2.
A novel avian β-defensin (AvBD), AvBD10, was discovered in the liver and bone marrow tissues from Chinese painted quail (Coturnix chinensis) in the present study. The complete nucleotide sequence of quail AvBD10 contains a 207-bp open reading frame that encodes 68 amino acids. The quail AvBD10 was expressed widely in all the tissues from quails except the tongue, crop, breast muscle, and thymus and was highly expressed in the bone marrow. In contrast to the expression pattern of AvBD10 in tissues from quail, the chicken AvBD10 was expressed in all 21 tissues from the layer hens investigated, with a high level of expression in the kidney, lung, liver, bone marrow, and Harderian glands. Recombinant glutathione S-transferase (GST)-tagged AvBD10s of both quail and chicken were produced and purified by expression of the two cDNAs in Escherichia coli, respectively. In addition, peptide according to the respective AvBD10s sequence was synthesized, named synthetic AvBD10s. As expected, both recombinant GST-tagged AvBD10s and synthetic AvBD10s of quail and chicken exhibited similar bactericidal properties against most bacteria, including Gram-positive and Gram-negative forms. However, no significant bactericidal activity was found for quail recombinant GST-tagged AvBD10 against Salmonella choleraesuis or for chicken recombinant GST-tagged AvBD10 against Proteus mirabilis.
本研究在中华鹧鸪（ Coturnix chinensis ）的肝脏和骨髓组织中发现了一种新型禽 β-防御素（AvBD），AvBD10。鹧鸪 AvBD10 的完整核苷酸序列包含一个 207bp 的开放阅读框，编码 68 个氨基酸。鹧鸪 AvBD10 在除舌头、嗉囊、胸肌和胸腺以外的所有鹧鸪组织中广泛表达，在骨髓中高度表达。与 AvBD10 在鹧鸪组织中的表达模式相反，鸡 AvBD10 在研究的 21 种鸡组织中均有表达，在肾脏、肺、肝脏、骨髓和哈德腺中表达水平较高。分别通过表达两种 cDNA 在大肠杆菌中产生和纯化了重组谷胱甘肽 S-转移酶（GST）标记的鸭和鸡 AvBD10。此外，根据各自的 AvBD10 序列合成了肽，命名为合成 AvBD10s。正如预期的那样，鸭和鸡的重组 GST 标记的 AvBD10s 和合成的 AvBD10s 对大多数细菌都表现出相似的杀菌特性，包括革兰氏阳性和革兰氏阴性形式。然而，未发现鸭重组 GST 标记的 AvBD10 对猪霍乱沙门氏菌或鸡重组 GST 标记的 AvBD10 对奇异变形杆菌有明显的杀菌活性。
