Laboratoire de modélisation numérique OPPUS, Département de génie chimique et de génie biotechnologique, Université de Sherbrooke, Sherbrooke, Québec, Canada J1K2R1.
Biotechnol Bioeng. 2012 Aug;109(8):2025-38. doi: 10.1002/bit.24473. Epub 2012 Mar 5.
A mathematical model for cellulase production by Trichoderma reesei RUT-C30 grown in a cellulose medium with lactose as fed batch in an airlift reactor is proposed. To describe adequately the mass transfer between the air bubbles and the broth, it uses computational fluid dynamics (CFD) including multiphase Eulerian-Eulerian formulation, with a detailed description of the bubble size distribution through the use of the population balance model (PBM) and the class method (CM). The kinetics of the biomass growth is further coupled to the fluid flow conditions using partial differential equations for all the species involved, providing detailed information of important reactor conditions such as the distribution of oxygen, cellulose, and the shear stress within the reactor over the entire period of fermentation. Predicted results agree well with the available overall measurements for a typical fed-batch operation and detailed profiles of the predicted concentration fields are discussed from an engineering viewpoint.
提出了一种在气升式反应器中以纤维素为培养基、乳糖为分批补料的条件下，生产里氏木霉 RUT-C30 纤维素酶的数学模型。为了充分描述气泡和培养基之间的质量传递，该模型使用计算流体动力学（CFD），包括多相欧拉-欧拉公式，并通过使用颗粒平衡模型（PBM）和分类方法（CM）详细描述了气泡尺寸分布。通过使用偏微分方程对所有涉及的物质进行耦合，将生物量生长动力学进一步与流体流动条件相关联，为整个发酵过程中重要的反应器条件（如氧气、纤维素和反应器内的剪切应力分布）提供了详细信息。预测结果与典型分批补料操作的可用总体测量值吻合较好，并从工程角度讨论了预测浓度场的详细分布情况。
