Avadi M R, Sadeghi A M M, Mohamadpour Dounighi Naser, Dinarvand R, Atyabi F, Rafiee-Tehrani M
Faculty of Pharmacy, Azad University of Medical Sciences, Tehran, Iran.
ISRN Pharm. 2011;2011:860109. doi: 10.5402/2011/860109. Epub 2011 Jul 10.
Polymeric delivery systems based on nanoparticles have emerged as a promising approach for peroral insulin delivery. The aim of the present study was to investigate the release of insulin nanoparticulate systems and ex vivo studies. The nanoparticles were prepared by the ion gelation method. Particle size distribution, zeta potential, and polydispersity index of the nanoparticles were determined. It was found that the nanoparticles carried positive charges and showed a size distribution in the range of 170-200 nm. The electrostatic interactions between the positively charged group of chitosan and negatively charged groups of Arabic gum play an important role in the association efficiency of insulin in nanoparticles. In vitro insulin release studies showed an initial burst followed by a slow release of insulin. The mucoadhesion of the nanosystem was evaluated using excised rat jejunum. Ex vivo studies have shown a significant increase in absorption of insulin in the presence of chitosan nanoparticles in comparison with free insulin.
基于纳米颗粒的聚合物递送系统已成为口服胰岛素递送的一种有前景的方法。本研究的目的是研究胰岛素纳米颗粒系统的释放及进行体外研究。纳米颗粒通过离子凝胶法制备。测定了纳米颗粒的粒径分布、zeta电位和多分散指数。发现纳米颗粒带正电荷，粒径分布在170 - 200nm范围内。壳聚糖带正电荷基团与阿拉伯胶带负电荷基团之间的静电相互作用在胰岛素在纳米颗粒中的结合效率中起重要作用。体外胰岛素释放研究显示胰岛素先有一个初始的突释，随后缓慢释放。使用切除的大鼠空肠评估纳米系统的黏膜黏附性。体外研究表明，与游离胰岛素相比，壳聚糖纳米颗粒存在时胰岛素的吸收显著增加。
