ten Cate W J, Patterson K, Rarey K E
Department of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville.
Acta Otolaryngol. 1990 Sep-Oct;110(3-4):234-40. doi: 10.3109/00016489009122542.
The morphology of rat ampullar dark cells was studied in the absence of circulating adrenal hormones at two time intervals of 8-14 and 22-28 days. Five morphological parameters of dark cells were quantitatively determined: cell area per baseline length, basolateral membrane surface density, basolateral membrane length per baseline length, volume density of intercellular space and volume density of vacuoles. Significant statistical reduction of basolateral membrane length per baseline length was observed after an absence of circulating adrenal hormones for 8-14 days and 22-28 days compared to sham operated animals that served as control. The basolateral membrane surface density decreased significantly in the animals sacrificed 22-28 days following adrenalectomy compared to sham operated animals. Cell area per baseline length decreased and volume density of vacuoles increased at both time intervals following adrenalectomy; however, these differences were statistically insignificant. The volume density of intercellular space between dark cells was practically constant among the groups. Results of this study are similar to those reported for ion-regulating nephron cells, and support the hypothesis that cells involved in microhomeostasis of vestibular endolymph may at least be partly regulated by adrenal hormones.
在缺乏循环肾上腺激素的情况下，于8 - 14天和22 - 28天这两个时间间隔对大鼠壶腹暗细胞的形态进行了研究。定量测定了暗细胞的五个形态学参数：每基线长度的细胞面积、基底外侧膜表面密度、每基线长度的基底外侧膜长度、细胞间隙体积密度和液泡体积密度。与作为对照的假手术动物相比，在缺乏循环肾上腺激素8 - 14天和22 - 28天后，观察到每基线长度的基底外侧膜长度有显著的统计学降低。与假手术动物相比，肾上腺切除术后22 - 28天处死的动物中基底外侧膜表面密度显著降低。肾上腺切除术后两个时间间隔，每基线长度的细胞面积均减少，液泡体积密度均增加；然而，这些差异在统计学上不显著。暗细胞之间的细胞间隙体积密度在各组之间实际上是恒定的。本研究结果与报道的离子调节肾单位细胞的结果相似，并支持这样的假说，即参与前庭内淋巴微内环境稳定的细胞可能至少部分受肾上腺激素调节。
