Portmann D, Fayad J, Linthicum F H, Rask-Andersen H
House Ear Institute, Los Angeles, California.
Acta Otolaryngol Suppl. 1990;470:7-12. doi: 10.3109/00016488909138349.
A technique to re-embed celloidin sections of human temporal bones for transmission electron microscopy (TEM) is presented. The procedure consists of basically four different steps, starting with loosening of the celloidin sections from the glass slides, removing the celloidin using clove oil, to staining and embedding in epoxy resin. The technique allows TEM analysis of the intact labyrinth in various regions in the same specimen, as well as on the same section. This may prove useful, as prior dissection of the fragile tissue components of the membranous labyrinth is not longer necessary. The technique makes possible the retrospective study of temporal bone collections. It may also be of value for the study of the ultrastructural histopathology of the human inner ear in optimally preserved tissue.
本文介绍了一种用于透射电子显微镜（TEM）的重新包埋人类颞骨火棉胶切片的技术。该过程主要包括四个不同步骤，首先是将火棉胶切片从载玻片上松开，然后用丁香油去除火棉胶，接着进行染色并嵌入环氧树脂。该技术允许对同一标本的不同区域以及同一切片上的完整迷路进行TEM分析。这可能很有用，因为不再需要事先解剖膜迷路的脆弱组织成分。该技术使对颞骨标本进行回顾性研究成为可能。对于在保存最佳的组织中研究人类内耳的超微结构组织病理学也可能具有价值。
