Laboratory of Food Analysis, Ghent University, Gent, Belgium.
Anal Bioanal Chem. 2012 Apr;403(1):265-78. doi: 10.1007/s00216-012-5803-3. Epub 2012 Mar 6.
Two multi-analyte flow-through immunoassay formats for rapid detection of mycotoxins in a variety of food matrices (peanut cake, maize, and cassava flour) were developed and evaluated. The selected food matrices are typical staple foods and export products for most low-income communities around the world. The assay formats included gel-based and membrane-based flow-through assays and were based on the principle of indirect enzyme-linked immunosorbent assay. Using the same immunoreagents, the performance characteristics of both assays were compared. To the best of our knowledge, this is the first report on such a comparison. The gel-based format was developed to screen for ochratoxin A, fumonisin B(1), deoxynivalenol, and zearalenone detection at cut-off values of 3, 1,250, 1,000, and 200 μg kg(-1), respectively, while the membrane-based format can be used to screen ochratoxin A, aflatoxin B(1,) deoxynivalenol, and zearalenone at the following cut-offs: 3, 5, 700, and 175 μg kg(-1), respectively. The applicability of these assay formats was demonstrated by evaluating the performance characteristics of both tests through performing multiple experiments on different days. Both assays were further evaluated by analyzing naturally contaminated samples in the laboratory and also in the field under tropical conditions (Cameroon, West Africa). The false-negative rate with both formats was less than 5%, which is in good agreement with Commission Decision 2002/657/EC regarding the performance of analytical methods intended for screening purposes.
两种多分析物流动注射免疫分析格式已被开发并用于快速检测多种食品基质（花生饼、玉米和木薯粉）中的霉菌毒素。所选的食品基质是世界上大多数低收入社区的典型主食和出口产品。分析格式包括基于凝胶和基于膜的流动注射分析，其原理基于间接酶联免疫吸附测定。使用相同的免疫试剂，比较了两种分析的性能特征。据我们所知，这是首次对此类比较进行的报告。基于凝胶的格式旨在筛选赭曲霉毒素 A、伏马菌素 B(1)、脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮，其截止值分别为 3、1,250、1,000 和 200 μg kg(-1)，而基于膜的格式可用于筛选赭曲霉毒素 A、黄曲霉毒素 B(1)、脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮，其截止值分别为 3、5、700 和 175 μg kg(-1)。通过在不同日期进行多次实验来评估两种测试的性能特征，证明了这些分析格式的适用性。在实验室和热带条件下（喀麦隆，西非）对自然污染的样品进行分析，进一步评估了这两种分析方法。两种格式的假阴性率均低于 5%，这与委员会关于用于筛选目的的分析方法性能的 2002/657/EC 号决定一致。
