[大鼠局灶性脑挫伤后荷马蛋白的表达]
[Homer protein expression after focal brain contusion in rats].
作者信息
Wang Fu-Yuan, Li Ru-Bo, Liang Hong-Xia, Shen Yu, Guo Xiao-Chong, Wang Zheng-Yin
机构信息
Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China.
出版信息
Fa Yi Xue Za Zhi. 2011 Dec;27(6):409-12.
OBJECTIVE
To investigate Homer protein expression after focal brain contusion and explore the relationship between expression and injury time.
METHODS
Focal brain contusion in rats was established and Homer protein expression in brain at different injury intervals after contusion was detected by immunohistochemistry and Western blotting.
RESULTS
A small amount of Homer positive expression cells were detected in control group, sham operated group and experimental group (0.5 h after contusion). The amount of Homer positive expression cells increased after 3 h and reached peak 12 h after contusion. The amount of positive cells continued to decrease 1 d after contusion and to the base level 7 d after contusion. Homer protein expression based on immunohistochemistry and Western blotting had statistical difference among adjacent groups.
CONCLUSION
Expression of Homer protein near the focal contusion area shows time dependence after brain contusion in rats.
目的
研究局灶性脑挫伤后Homer蛋白的表达情况，并探讨其表达与损伤时间的关系。
方法
建立大鼠局灶性脑挫伤模型，采用免疫组织化学和蛋白质印迹法检测挫伤后不同损伤时间点脑组织中Homer蛋白的表达。
结果
对照组、假手术组及实验组（挫伤后0.5小时）均检测到少量Homer阳性表达细胞。挫伤后3小时Homer阳性表达细胞数量增加，12小时达到峰值。挫伤后1天阳性细胞数量持续减少，并在挫伤后7天降至基础水平。基于免疫组织化学和蛋白质印迹法的Homer蛋白表达在相邻组间具有统计学差异。
结论
大鼠脑挫伤后，局灶性挫伤区域附近Homer蛋白的表达呈时间依赖性。
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