Liu Hongcheng, Li Qiwan, Shao Jinliang, Yan Hongmei, Lan Shanshan
Institute of Quality Standard and Testing Technology, Yunnan Academy of Agricultural Sciences, Kunming 650223, China.
Se Pu. 2011 Nov;29(11):1103-6.
A method of high performance liquid chromatography was established for the analysis of trigonelline in coffee powder and instant coffee. The separation was performed on a BondPak NH2 column (250 mm x 4.6 mm, 5 microm). The mobile phase was methanol-water (82: 18, v/v) at a flow rate of 0.8 mL/min. The detection wavelength was set at 260 nm. The method had good linearity in the range of 1 - 40 mg/L (the correlation coefficient was 0.9998). The repeatability of the method was performed at one day, different days, and by two analysts. The accuracy of the method was evaluated by recovery study. The recoveries were more than 90% with the relative standard deviations less than 3%. The contents of trigonelline in samples were assessed with ultrasonic extraction and hot water extraction, separately, and the regression coefficient between trigonelline contents obtained under the two extraction methods was 0.9964. The method is simple, rapid, highly sensitive, and suitable for the analysis of trigonelline and quality control of coffee samples.
建立了一种高效液相色谱法用于分析咖啡粉和速溶咖啡中的胡芦巴碱。分离在BondPak NH2柱（250 mm×4.6 mm，5μm）上进行。流动相为甲醇 - 水（82:18，v/v），流速为0.8 mL/min。检测波长设定为260 nm。该方法在1 - 40 mg/L范围内具有良好的线性（相关系数为0.9998）。该方法的重复性在同一天、不同天以及由两名分析人员进行测试。通过回收率研究评估该方法的准确性。回收率超过90%，相对标准偏差小于3%。分别用超声提取和热水提取法测定样品中胡芦巴碱的含量，两种提取方法所得胡芦巴碱含量之间的回归系数为0.9964。该方法简便、快速、灵敏度高，适用于胡芦巴碱的分析和咖啡样品的质量控制。
