Beclin 1对人宫颈癌细胞中血管生成素及Tie-2受体表达的调节作用
Modulatory effects of Beclin 1 on expression of angiopoietin and Tie-2 receptor in human cervical cancer cells.
作者信息
Sun Yang, Liu Jia-hua, Pan Ling, Jin Long, Yang Yin, Sui Yu-xia, Shi Hong
机构信息
Department of Obstetrics and Gynecology, Fujian Provincial Hospital, Fujian Provincial Clinical Medical College, Fujian Medical University, Fuzhou, China.
出版信息
Asian Pac J Cancer Prev. 2011;12(11):2985-90.
AIM
To investigate the effect of Beclin 1, an autophagy gene, on the expression of angiopoietin (Ang) protein and the Tie-2 receptor in CaSki human cervical cancer cells.
MATERIALS AND METHODS
Beclin 1 overexpression (pcDNA3.1-Beclin1) and knockdown (pSUPER-Beclin1) plasmids were independently transfected into CaSki cells, and stably transfected cells were selected. Real-time fluorescence quantitative PCR and Western blot analyses were performed to detect the mRNA and protein expression of Beclin 1, Ang-1, Ang-2, and Tie-2. MTT assays were employed to determine cell proliferation rates.
RESULTS
In the cells transfected with pcDNA3.1-Beclin1, the expression of Beclin 1, Ang-2, and Tie-2 was markedly increased, but expression of Ang-1 was dramatically reduced. MTT assays revealed that the proliferation of these cells was also significantly suppressed. In the CaSki cells transfected with pSUPER-Beclin1, the expression of Beclin 1, Ang-2, and Tie-2 was inhibited.
CONCLUSION
Overexpression of Beclin 1 can inhibit the proliferation of CaSki cells, which may be attributed to an imbalance among the expression of Ang-1, Ang-2 and Tie-2.
目的
研究自噬基因Beclin 1对人宫颈癌CaSki细胞中血管生成素（Ang）蛋白及Tie-2受体表达的影响。
材料与方法
将Beclin 1过表达质粒（pcDNA3.1-Beclin1）和敲低质粒（pSUPER-Beclin1）分别转染至CaSki细胞中，并筛选出稳定转染的细胞。采用实时荧光定量PCR和蛋白质印迹分析检测Beclin 1、Ang-1、Ang-2及Tie-2的mRNA和蛋白表达。运用MTT法测定细胞增殖率。
结果
在转染pcDNA3.1-Beclin1的细胞中，Beclin 1、Ang-2及Tie-2的表达显著增加，但Ang-1的表达显著降低。MTT分析显示这些细胞的增殖也受到显著抑制。在转染pSUPER-Beclin1的CaSki细胞中，Beclin 1、Ang-2及Tie-2的表达受到抑制。
结论
Beclin 1的过表达可抑制CaSki细胞的增殖，这可能归因于Ang-1、Ang-2和Tie-2表达失衡。
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