Chen Guo-qing, Zhang Hua, Qi Hong-bo, Yao Zhen-wei, Gao Li, Qiu Chun-ling
Department of Obstetrics and Gynecology, Chongqing Medical University, Chongqing, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Mar;28(3):294-6.
To investigate the occurrence law of autophagy in trophoblast cells from severe preeclampsia and its possible mechanisms of wrecking cells.
23 cases of placenta tissues were collected from women suffer from severe preeclampsia and normal pregnant women respectively. Surface villous of placental was observed by scanning electron microscope, and ultrastructure and autophagosome of trophoblast cells were observed by transmission electron microscopic. The expressions of Beclin-1 and LC3II in placenta tissues were detected by Immunohistochemical technique, then its relevance whit weight of fetus and placental was analyzed.
Compared with the control group, villous were decrease in placenta tissue from patients suffer from severe preeclampsia, and its arrangement was disordered; typical autophagosomes of trophoblast cells were observed by transmission electron microscope. The expression of Beclin-1, LC3IIwere significantly up-regulated in placenta tissue from patients suffer from severe preeclampsia.The correlation coefficient of Beclin-1, LC3II and placental weight, fetal weight respectively were -0.977, -0.930, -0.812, -0.849.
Autophagy were significantly up-regulated in trophoblast cells from patients suffer from severe preeclampsia by increasing expression of Beclin-1, LC3II protein, and its expression was negatively related to weight of fetus and placental.
探讨重度子痫前期患者滋养层细胞自噬的发生规律及其可能的细胞破坏机制。
分别收集23例重度子痫前期患者及正常孕妇的胎盘组织。用扫描电子显微镜观察胎盘表面绒毛，用透射电子显微镜观察滋养层细胞的超微结构及自噬体。采用免疫组织化学技术检测胎盘组织中Beclin-1和LC3II的表达，然后分析其与胎儿及胎盘重量的相关性。
与对照组相比，重度子痫前期患者胎盘组织中的绒毛减少，排列紊乱；透射电子显微镜观察到滋养层细胞有典型的自噬体。重度子痫前期患者胎盘组织中Beclin-1、LC3II的表达显著上调。Beclin-1、LC3II与胎盘重量、胎儿重量的相关系数分别为-0.977、-0.930、-0.812、-0.849。
重度子痫前期患者滋养层细胞的自噬通过增加Beclin-1、LC3II蛋白的表达而显著上调，且其表达与胎儿及胎盘重量呈负相关。
