Dragomir L P, Simionescu Cristiana, Mărgăritescu Cl, Stepan A, Dragomir Iuliana Manuela, Popescu M R
Department of Occlusology Dental Prosthetics, University of Medicine and Pharmacy of Craiova, Romania.
Rom J Morphol Embryol. 2012;53(1):89-93.
In this study, we have analyzed clinically, histopathologically and immunohistochemically a total of 34 cases of oral squamous carcinoma in 11 of the cases being identified adjacent epithelial dysplastic lesions. Carcinomas were diagnosed in patients aged 40-60 years, males, with chronic exposure to tobacco and/or alcohol, being located especially on the lips. Well-differentiated carcinomas have been predominant (52.9%) in stage I/II tumoral (88.3%). Immunoexpression analysis of p53, p16 and Ki67 did not reveal statistically significant differences between the expression of markers and clinical or histopathological parameters, except Ki67 whose increased expression was associated to the decrease of the degree of tumoral differentiation and with high degree dysplasia. The positivity index and the intensity of reaction were increased at the level of dysplasic epithelium for p16 and at the level of tumoral invasion front for the p53 and Ki67. The study highlights the value of the immunostain for p16 in identifying dysplasic lesions and predictive importance of p53 and Ki67 markers in identifying the aggressive forms of oral carcinomas.
在本研究中，我们对34例口腔鳞状细胞癌进行了临床、组织病理学和免疫组织化学分析，其中11例伴有相邻上皮发育异常病变。这些癌患者年龄在40至60岁之间，为男性，长期接触烟草和/或酒精，尤其发生于唇部。高分化癌在I/II期肿瘤中占主导（52.9%）（88.3%）。p53、p16和Ki67的免疫表达分析显示，除Ki67外，标志物表达与临床或组织病理学参数之间无统计学显著差异，Ki67表达增加与肿瘤分化程度降低及高度发育异常相关。p16在发育异常上皮水平的阳性指数和反应强度增加，p53和Ki67在肿瘤浸润前沿水平增加。该研究突出了p16免疫染色在识别发育异常病变中的价值以及p53和Ki67标志物在识别侵袭性口腔癌形式中的预测重要性。
