Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Endocrine. 2012 Oct;42(2):329-34. doi: 10.1007/s12020-012-9630-2. Epub 2012 Mar 7.
This study aimed to examine the expression of cholecystokinin-1 receptor (CCK-1R) in the kidneys of type 2 diabetic nephropathy (DN) and the correlation of CCK-1R mRNA and proteinuria. Localization of CCK-1R in kidney of diabetic patient with nephropathy was examined by immunohistochemistry and in situ hybridization. The glomeruli did not express CCK-1R in either control or diabetic nephropathic kidneys. However, the expressions of CCK-1R protein and mRNA in tubules were significantly increased in DN, which had no relationship with the severity of DN. Furthermore, there was a positive correlation between the percentage of cells positive for CCK-1R mRNA and the degree of proteinuria. Increased CCK-1R expression could be demonstrated in the tubules and the CCK-1R might be implicated in the development of proteinuria in human DN.
本研究旨在探讨胆囊收缩素 1 型受体（CCK-1R）在 2 型糖尿病肾病（DN）患者肾脏中的表达及其与蛋白尿的相关性。采用免疫组化和原位杂交技术检测糖尿病肾病患者肾脏中 CCK-1R 的定位。结果发现，在对照组和糖尿病肾病患者的肾小球中均未表达 CCK-1R。然而，在 DN 中，肾小管中 CCK-1R 蛋白和 mRNA 的表达显著增加，与 DN 的严重程度无关。此外，CCK-1R mRNA 阳性细胞百分比与蛋白尿程度呈正相关。在肾小管中可以观察到 CCK-1R 表达增加，CCK-1R 可能参与了人类 DN 蛋白尿的发生。
