Department of Orthodontics, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
Acta Odontol Scand. 2012 Jul;70(4):265-71. doi: 10.3109/00016357.2011.641585. Epub 2012 Mar 8.
Root resorption, impaired tooth eruption and early tooth loss have been described in relation to diseases that involve defects in the RANK-RANKL-OPG-expression. The aim of the present immunhistochemical study was to localize and compare the reactions for RANK and membrane-bound RANKL along root surfaces and in the periodontal membrane in close proximity to the root surface of human primary and permanent teeth.
The material comprised extracted human teeth (11 primary teeth and six permanent teeth) from 10 different patients. Paraffin sections were prepared of each tooth and sections of each tooth were immunohistochemically stained with antibodies specific for membrane-bound RANKL and RANK.
The root surface and the periodontal membrane in close proximity to the root surface did not show immunoreactivity for RANKL. RANKL was only located in odontoblasts and in cells along denticles in one primary tooth. RANK was located in mononuclear cells in the pulp and in multinucleated odontoclasts along resorbed root surfaces and along resorbed dentin surfaces in the pulp in primary teeth and one permanent tooth.
This study demonstrated RANK positivity in resorption areas in primary and permanent teeth. RANKL was positive in the pulp of one primary tooth. RANK expression in odontoclasts and RANKL expression in the pulp may indicate that RANK/RANKL play a role during resorption.
与涉及 RANK-RANKL-OPG 表达缺陷的疾病相关，已经描述了牙根吸收、牙齿萌出受损和早期牙齿脱落。本免疫组织化学研究的目的是定位和比较人乳牙和恒牙根表面和根表面附近牙周膜中 RANK 和膜结合型 RANKL 的反应。
该材料包括来自 10 名不同患者的 11 颗乳牙和 6 颗恒牙。对每颗牙齿进行石蜡切片，并使用针对膜结合型 RANKL 和 RANK 的抗体对每颗牙齿的切片进行免疫组织化学染色。
根表面和根表面附近的牙周膜没有 RANKL 的免疫反应性。RANKL 仅位于一颗乳牙的成牙本质细胞和成牙本质细胞中。RANK 位于牙髓中的单核细胞和沿吸收根表面和牙髓中吸收牙本质表面的多核破牙细胞中。
本研究表明 RANK 在乳牙和恒牙的吸收区域呈阳性。RANKL 在一颗乳牙的牙髓中呈阳性。破牙细胞中的 RANK 表达和牙髓中的 RANKL 表达可能表明 RANK/RANKL 在吸收过程中发挥作用。
