He Yang, Hou Pei, Fan Gang, Song Zhen, Arain Saima, Shu Hao, Tang Ce, Yue Qinghong, Zhang Yi
College of Ethnomedicines, College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P.R. China.
Mitochondrial DNA. 2012 Apr;23(2):100-5. doi: 10.3109/19401736.2012.660924. Epub 2012 Mar 7.
Angelica anomala Avé-Lall (Chuanbaizhi in Chinese) is an important medicinal plant which can be used in traditional Chinese medicines; however, there are no authentic and universal methods to differentiate this Sichuan famous-region drug of A. anomala from a large number of non-famous-region and false drugs. It has been demonstrated that DNA barcoding is a molecular diagnostic method for species identification, which uses a single standardized DNA fragment. In this study, we tested five DNA barcoding candidates (matK, ITS, ITS2, rbcL, and psbA-trnH), and we found that ITS was the best candidate to authenticate the famous-region drug of A. anomala. Moreover, through comparative analysis of these five DNA barcodes between A. anomala and Angelica dahurica, we found that ITS had the most and ITS2 had more variable regions, but the psbA-trnH, rbcL, and matK regions were identical. Hence, we suggest ITS as the DNA barcoding to identify A. anomala and A. dahurica. Moreover, we are determined to adopt the A. anomala as the accurate Latin name of Chuanbaizhi.
拐芹当归（中药名：川白芷）是一种重要的药用植物，可用于传统中药；然而，目前尚无可靠且通用的方法将这种四川道地药材拐芹当归与大量非道地及伪品区分开来。已有研究表明，DNA条形码技术是一种利用单个标准化DNA片段进行物种鉴定的分子诊断方法。在本研究中，我们测试了五个DNA条形码候选序列（matK、ITS、ITS2、rbcL和psbA-trnH），发现ITS是鉴定拐芹当归道地药材的最佳候选序列。此外，通过对拐芹当归和白芷这五个DNA条形码的比较分析，我们发现ITS的可变区最多，ITS2的可变区较多，而psbA-trnH、rbcL和matK区域则完全相同。因此，我们建议将ITS作为鉴定拐芹当归和白芷的DNA条形码。此外，我们决定采用拐芹当归作为川白芷准确的拉丁学名。
