USDA, Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, Crop Diseases, Pests and Genetics Unit, Parlier, CA 93648, USA.
Mol Plant Microbe Interact. 2012 Jun;25(6):747-54. doi: 10.1094/MPMI-11-10-0270.
The bacterium Xylella fastidiosa causes a number of plant diseases of significant economic impact. To date, progress determining mechanisms of host-plant susceptibility, tolerance, or resistance has been slow, due in large part to the long generation time and limited available genetic resources for grape, almond, and other known hosts of X. fastidiosa. To overcome many of these limitations, Arabidopsis thaliana has been evaluated as a host for X. fastidiosa. A pin-prick inoculation method has been developed to infect Arabidopsis with X. fastidiosa. Following infection, X. fastidiosa multiplies and can be detected by microscopy, polymerase chain reaction, and isolation. The ecotypes Van-0, LL-0, and Tsu-1 all allow more growth of strain X. fastidiosa Temecula than the reference ecotype Col-0. Affymetrix ATH1 microarray analysis of inoculated vs. noninoculated Tsu-1 reveals gene expression changes that differ greatly from changes seen after infection with apoplast-colonizing bacteria such as Psuedomonas syringae pvs. tomato or syringae. Many genes responsive to oxidative stress are differentially regulated, while classic pathogenesis-related genes are not induced by X. fastidiosa infection.
韧皮部杆菌 Xylella fastidiosa 可引起多种对经济有重大影响的植物疾病。迄今为止，由于葡萄、杏仁和其他已知 X. fastidiosa 宿主的世代时间长且可用的遗传资源有限，确定宿主植物易感性、耐受性或抗性机制的进展一直很缓慢。为了克服这些限制中的许多限制，拟南芥已被评估为 X. fastidiosa 的宿主。已经开发了一种针刺接种方法来用 X. fastidiosa 感染拟南芥。感染后，X. fastidiosa 会繁殖，可以通过显微镜、聚合酶链反应和分离来检测。生态型 Van-0、LL-0 和 Tsu-1 都比参考生态型 Col-0 允许更多的 Temecula 菌株 X. fastidiosa 生长。接种与未接种 Tsu-1 的 Affymetrix ATH1 微阵列分析显示，与感染质外体定殖细菌（如番茄假单胞菌 pv. 或丁香假单胞菌）后的变化相比，基因表达变化差异很大。许多对氧化应激有反应的基因受到差异调控，而经典的病程相关基因不受 X. fastidiosa 感染的诱导。
