Department of Clinical Biochemistry, Faculty of Health Sciences Bispebjerg Hospital, Copenhagen, Denmark.
Scand J Clin Lab Invest. 2012 Jul;72(4):287-95. doi: 10.3109/00365513.2012.662281. Epub 2012 Mar 8.
To evaluate the influence of time of day on the circulating concentrations of 14 frequently used clinical biochemical parameters in the Bispebjerg study of diurnal variations.
Venous blood samples were obtained under controlled environmental, activities and food conditions from 24 healthy young men every third hour through 24 hours, nine time points in total. At each time point, the parameters' concentrations were measured. The data were analyzed by rhythmometric statistical methods and in addition the biological variations were calculated.
Significant oscillation of melatonin with an amplitude (amp) of 19.84 pg/ml and a peak at 03:34 h confirmed the normal 24-hour rhythms of the participants. Potassium (p < 0.0001, amp = 0.18 mmol/L), sodium (p < 0.0001, amp = 1.10 mmol/L) creatine kinase (p = 0.01, amp = 17.18 U/L), bilirubin (p < 0.0001, amp = 2.36 μmol/L) and aspartate aminotransferase (p < 0.0001, amp = 1.66 U/L) oscillated with gradually falling mean concentrations through the day to nadir around midnight. Urea nitrogen (p = 0.01, amp = 0.22 mmol/L) oscillated with gradually increasing mean concentrations through the day peaking around midnight. Lactate dehydrogenase (p < 0.0001, amp = 9.76 U/L) oscillated with gradually increasing concentrations through the early day peaking in the afternoon. Uric acid (p = 0.03, amp = 0.013 mmol/L) oscillated with gradually increasing concentrations through the night peaking in the morning. Potassium and sodium had the highest 24-hour oscillations in proportion to the reference intervals of the parameters for healthy young men.
In the clinical setting, diurnal variations of clinical biochemical parameters commonly used through the day and night must be considered when concentration changes in the parameters are evaluated especially potassium and sodium.
评估时间对 14 种常用临床生化参数在比斯加夫研究昼夜节律中循环浓度的影响。
在受控环境、活动和饮食条件下，从 24 名健康年轻男性中每三小时采集一次静脉血样，总共采集 24 小时，9 个时间点。在每个时间点，测量参数的浓度。数据通过节奏统计方法进行分析，并计算了生物学变异。
证明参与者的正常 24 小时节律的褪黑素的显著振荡，幅度（amp）为 19.84pg/ml，峰值为 03:34h。钾（p<0.0001，amp=0.18mmol/L）、钠（p<0.0001，amp=1.10mmol/L）、肌酸激酶（p=0.01，amp=17.18U/L）、胆红素（p<0.0001，amp=2.36μmol/L）和天门冬氨酸氨基转移酶（p<0.0001，amp=1.66U/L）在白天逐渐降低的平均浓度下波动，午夜达到最低值。尿素氮（p=0.01，amp=0.22mmol/L）在白天逐渐增加的平均浓度下波动，午夜达到峰值。乳酸脱氢酶（p<0.0001，amp=9.76U/L）在白天逐渐增加的浓度下波动，下午达到峰值。尿酸（p=0.03，amp=0.013mmol/L）在夜间逐渐增加的浓度下波动，清晨达到峰值。钾和钠的 24 小时波动幅度最大，与健康年轻男性参数参考区间的比例最高。
在临床环境中，当评估参数浓度变化时，必须考虑常用的临床生化参数昼夜变化，尤其是钾和钠。
