Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, India.
Anim Reprod Sci. 2012 Mar;131(1-2):54-62. doi: 10.1016/j.anireprosci.2012.02.003. Epub 2012 Feb 11.
A study was carried out to assess the effect of sequestration of PDC-109 protein, a majority constituent of heparin binding proteins (HBP) of seminal plasma, on freezability and in vitro fertilizing ability of crossbred bull spermatozoa after cryopreservation. The study consisted of isolation and characterization of PDC-109 protein to raise anti-sera against it in rabbits. Following which, raised antibodies against PDC-109 protein was quantitated and coated in tubes used for collection of ejaculates. Semen ejaculates thus collected were cryopreserved using EYTG extender. Physico-morphological characteristics, viz. motility, viability, acrosomal integrity and HOS response as an indicator of freezability of cryopreserved spermatozoa were determined at pre freeze as well as post thaw stage. At pre freeze stage, a significant (p<0.05) improvement in viability (83.83 ± 2.18 vs 75.17 ± 2.42) and acrosome integrity (81.33 ± 2.38 vs 72.83 ± 2.39) in antibodies treated group than control was observed. Similarly, increase in HOS responsive spermatozoa was highly significant (p<0.01) than control (78.83 ± 1.69 vs 67.5 ± 1.75). At post thaw stage, significant (p<0.05) improvement in viability (69.50 ± 2.16 vs 60.33 ± 2.19) and HOS responsive spermatozoa (68.67 ± 1.62 vs 58.50 ± 1.32) and highly significant (p<0.01) increase in individual motility (56.17 ± 1.83 vs 47.00 ± 1.86) and acrosome integrity (75.17 ± 2.38 vs 61.83 ± 2.1) was observed in antibodies treated group when compared to control was observed. The results from the study revealed that sequestration of PDC-109 protein from semen samples leads to significant improvement in pre-freeze and post-thaw values of above parameters in cryopreserved spermatozoa. It is thus concluded that sequestration of PDC-109 protein from ejaculates improves freezability of crossbred bull spermatozoa.
一项研究旨在评估精液肝素结合蛋白（HBP）主要成分 PDC-109 蛋白的隔离对冷冻保存后杂交公牛精子的可冻性和体外受精能力的影响。该研究包括 PDC-109 蛋白的分离和特性鉴定，以在兔子中产生针对它的抗血清。随后，定量检测针对 PDC-109 蛋白的抗体，并将其涂覆在用于收集精液的管中。使用 EYTG extender 冷冻保存收集到的精液。在冷冻前和解冻后阶段，分别测定冷冻保存精子的物理形态特征，即活力、活力、顶体完整性和 HOS 反应作为可冻性的指标。在冷冻前阶段，与对照组相比，抗体处理组的活力（83.83 ± 2.18 vs 75.17 ± 2.42）和顶体完整性（81.33 ± 2.38 vs 72.83 ± 2.39）显著提高（p<0.05）。同样，HOS 反应性精子的增加也非常显著（p<0.01），比对照组高（78.83 ± 1.69 vs 67.5 ± 1.75）。在解冻后阶段，与对照组相比，活力（69.50 ± 2.16 vs 60.33 ± 2.19）和 HOS 反应性精子（68.67 ± 1.62 vs 58.50 ± 1.32）以及个体活力（56.17 ± 1.83 vs 47.00 ± 1.86）和顶体完整性（75.17 ± 2.38 vs 61.83 ± 2.1）的显著改善（p<0.05）和非常显著（p<0.01）均观察到在抗体处理组中。研究结果表明，从精液样本中隔离 PDC-109 蛋白可显著提高冷冻保存精子的冷冻前和冷冻后以上参数的值。因此，可以得出结论，从精液中隔离 PDC-109 蛋白可提高杂交公牛精子的可冻性。
