Fratantoni J C, Poindexter B J
Division of Blood and Blood Products, Center for Biologics Evaluation and Research, Bethesda, Maryland 20892.
Am J Clin Pathol. 1990 Nov;94(5):613-7. doi: 10.1093/ajcp/94.5.613.
Platelet aggregation measurements were done with the use of a commercially available microtiter plate reader with specific modification of the mode of agitation of the samples. Satisfactory aggregation curves were obtained with use of an external horizontal agitator, with an amplitude of 1.3 mm and minimum frequency of 1,360 cycles/minute. With the use of the 96 available wells in the microtiter plates, all test and control platelet samples, with replicates, were observed simultaneously and the output data obtained within 10-15 minutes. The technique was validated by demonstrating the similarity of dose-response curves, obtained with a standard aggregometer and with the microtiter technique, of platelets stimulated by adenosine diphosphate, thrombin, and arachidonic acid.
血小板聚集测量是使用市售的酶标仪进行的，对样品的搅拌模式进行了特定修改。通过使用外部水平搅拌器，振幅为1.3毫米，最低频率为1360次/分钟，获得了令人满意的聚集曲线。利用酶标板中的96个可用孔，同时观察所有测试和对照血小板样品及其复制品，并在10 - 15分钟内获得输出数据。通过证明用标准凝集仪和微量滴定技术获得的由二磷酸腺苷、凝血酶和花生四烯酸刺激的血小板的剂量反应曲线的相似性，验证了该技术。
