Department of Chemistry, University of California-Davis, Davis, CA 95616, United States.
Colloids Surf B Biointerfaces. 2012 Jun 1;94:374-7. doi: 10.1016/j.colsurfb.2012.01.034. Epub 2012 Feb 10.
Attenuated total reflection Fourier transform infrared spectroscopy was used to monitor the formation of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), DMPC: lactosylceramide, and DMPC: GD3 lipid bilayers onto a zinc selenide surface. Infrared absorption peak position, bandwidth, and intensity were all used to monitor the formation, acyl chain ordering, and chemical environment within each bilayer. The results from this study indicate that the addition of glycosphingolipids into a DMPC lipid bilayer introduces decreases in both, acyl chain ordering, and homogeneity within the bilayer. GD3:DMPC lipid bilayers possess lipid chain characteristics that are indiscernible from those present in the lactosylceramide:DMPC bilayer, while possessing different structural head groups, indicating that the head group has little influence on the underlying lipid structure. Differences in the phosphate hydration are, however, evident between the three types of bilayer, with phosphate hydration decreasing in the order LacCer:DMPC (1223.4 cm(-1))>DMPC only (1226 cm(-1))>GD3:DMPC (1229.6 cm(-1)).
衰减全反射傅里叶变换红外光谱被用于监测 1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）、DMPC：乳糖基神经酰胺和 DMPC：GD3 脂质双层在硒化锌表面上的形成。红外吸收峰位置、带宽和强度都被用来监测各双层的形成、酰基链有序性和化学环境。本研究的结果表明，糖脂的加入会降低 DMPC 脂质双层的酰基链有序性和双层内的均一性。GD3:DMPC 脂质双层的脂质链特征与乳糖基神经酰胺：DMPC 双层中的特征难以区分，而具有不同的结构头部基团，这表明头部基团对底层脂质结构的影响很小。然而，三种类型的双层之间的磷酸盐水合作用存在明显差异，磷酸盐水合作用的顺序为 LacCer:DMPC（1223.4 cm(-1))>仅 DMPC（1226 cm(-1))>GD3:DMPC（1229.6 cm(-1))。
