Laboratoire de Virologie EA3610, Université Lille 2, Faculté de Médecine, CHRU Lille, Lille, France.
Intervirology. 2012;55(6):435-41. doi: 10.1159/000335549. Epub 2012 Mar 6.
Type B coxsackieviruses (CV-B), together with echoviruses (E), are among the most common pathogens encountered in aseptic meningitis and meningoencephalitis. They frequently infect the central nervous system (CNS). The mechanisms of virus spreading in the CNS are poorly understood. In the present study, we investigated CV-B4 and E-9 spreading and neurotropism within intraperitoneally inoculated one-week-old Swiss albino mice.
Seminested RT-PCR and virus isolation were used to assay viral distribution.
Viral RNA was present in various organs: brain, spinal cord, spleen and heart at various times post-infection (p.i.); ranging from 1 day p.i. up to 30, 60 and 90 days p.i, respectively, for CV-B4-JVB-, E-9 Barty- and CV-B4-E2-infected mice. Organs became negative for virus isolation after 5 days p.i., except for brain and heart from CV-B4 E2-infected mice, which remained positive for up to 10 and 15 days p.i., respectively. Negative viral RNA strand was detected mainly in brain and spinal cord of infected mice until 30 and 60 days p.i.
This is the first report on the persistence of CV-B4 and E-9 in the CNS of intraperitoneally inoculated mice.
B 型柯萨奇病毒（CV-B）与埃可病毒（E）一起是无菌性脑膜炎和脑膜脑炎中最常见的病原体之一。它们经常感染中枢神经系统（CNS）。病毒在中枢神经系统中传播的机制尚不清楚。在本研究中，我们研究了腹腔内接种一周龄瑞士白化小鼠体内 CV-B4 和 E-9 的传播和嗜神经性。
采用半巢式 RT-PCR 和病毒分离法检测病毒分布。
病毒 RNA 存在于各种器官中：脑、脊髓、脾和心，在感染后不同时间（p.i.）；分别为 CV-B4-JVB-、E-9 Barty-和 CV-B4-E2 感染小鼠的 1 天 p.i.至 30、60 和 90 天 p.i.。感染后 5 天 p.i.，除 CV-B4 E2 感染小鼠的脑和心外，所有器官的病毒分离均呈阴性，这些器官的阳性结果分别持续至 10 和 15 天 p.i.。在感染小鼠的脑和脊髓中主要检测到负链病毒 RNA，直至 30 和 60 天 p.i.。
这是首例关于 CV-B4 和 E-9 在腹腔接种小鼠中枢神经系统中持续存在的报告。
