Department of Pathology, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan.
Hum Pathol. 2012 Aug;43(8):1322-7. doi: 10.1016/j.humpath.2011.10.013. Epub 2012 Mar 7.
Forkhead box P1 protein is a transcription factor involved in cell signaling and regulation of gene expression and is essential for B-cell development. Forkhead box P1 overexpression has been associated with a worsened prognosis in some B-cell lymphomas. However, little is known about the clinicopathologic significance of forkhead box P1 in T-cell malignancies. In this study, immunohistochemistry for forkhead box P1 was performed in peripheral T-cell lymphoma, not otherwise specified, cases (n = 41), which were then divided into lower (n = 15) and higher (n = 26) forkhead box P1 expressers. Results of real-time quantitative reverse transcriptase polymerase chain reaction for forkhead box P1 messenger RNA supported the data on immunohistochemical forkhead box P1 expression. Forkhead box P1 overexpression in lymphoma cells was inversely associated with proliferation activity as evaluated by Ki-67 expression. Double immunostain for forkhead box P1 and a T-cell marker in normal lymph nodes showed forkhead box P1 signals in many of nonneoplastic T cells. Prognostic analysis showed that forkhead box P1 overexpression was associated with an improved overall survival of the patients with peripheral T-cell lymphoma, not otherwise specified, and was independent of the International Prognostic Index in multivariate analysis. Forkhead box P1 overexpression may be associated with less activated phenotype of the tumors and with a better prognosis in patients with peripheral T-cell lymphoma, not otherwise specified. The clinicopathologic significance of forkhead box P1 overexpression in peripheral T-cell lymphoma, not otherwise specified, may be different from that in B-cell lymphomas.
叉头框蛋白 P1 是一种参与细胞信号转导和基因表达调控的转录因子，对 B 细胞发育至关重要。叉头框蛋白 P1 的过表达与一些 B 细胞淋巴瘤的预后恶化有关。然而，关于叉头框蛋白 P1 在 T 细胞恶性肿瘤中的临床病理意义知之甚少。在这项研究中，对未特指的外周 T 细胞淋巴瘤病例（n=41）进行了叉头框蛋白 P1 的免疫组织化学检测，然后将其分为低表达（n=15）和高表达（n=26）组。实时定量逆转录聚合酶链反应（real-time quantitative reverse transcriptase polymerase chain reaction）检测结果支持了免疫组化检测到的叉头框蛋白 P1 表达数据。淋巴瘤细胞中叉头框蛋白 P1 的过表达与 Ki-67 表达评估的增殖活性呈负相关。在正常淋巴结中，对叉头框蛋白 P1 和 T 细胞标志物进行双重免疫染色显示，许多非肿瘤性 T 细胞中存在叉头框蛋白 P1 信号。预后分析表明，外周 T 细胞淋巴瘤患者中叉头框蛋白 P1 的过表达与总生存率的提高相关，并且在多变量分析中独立于国际预后指数。叉头框蛋白 P1 的过表达可能与肿瘤的激活表型较少有关，并与未特指的外周 T 细胞淋巴瘤患者的预后较好相关。在未特指的外周 T 细胞淋巴瘤中，叉头框蛋白 P1 过表达的临床病理意义可能与 B 细胞淋巴瘤不同。
