Department of Chemistry, Moscow State University, 119991 Moscow, Leninskiye Gory 1/3, Russian Federation.
Peptides. 2012 Apr;34(2):296-302. doi: 10.1016/j.peptides.2012.02.017. Epub 2012 Mar 3.
Identification of species constituting Rana esculenta complex represents a certain problem as two parental species Rana ridibunda and Rana lessonae form their hybrid R. esculenta, while external signs and sizes of the members of this complex are intersected. However the composition of skin secretion consisting mainly of peptides is different for the species of the complex. LC-MS/MS is an ideal analytical tool for the quantitative and qualitative analysis of these peptides. The results covering elemental composition of these peptides, their levels in the secretion, as well as their belonging to a certain family of peptides may be visualized by means of 2D mass maps. The proposed approach proved itself to be a perspective tool for the reliable identification of all 3 species constituting R. esculenta complex. Easy distinguishing between the species may be achieved using 2D maps as fingerprints. Besides this approach may be used to study hybridogenesis and mechanisms of hemiclonal transfer of genetic information, when rapid and reliable identification of species involved in the process is required.
鉴定构成中华蟾蜍复合体的物种是一个难题，因为两个亲本物种花背蟾蜍和华西雨蛙形成了它们的杂交种中华蟾蜍，而这个复合体的成员的外部特征和大小是交叉的。然而，由主要由肽组成的皮肤分泌物的组成在复合体的物种之间是不同的。LC-MS/MS 是定量和定性分析这些肽的理想分析工具。通过二维质量图谱，可以直观地显示这些肽的元素组成、在分泌物中的水平以及它们属于某一种肽家族的情况。该方法已被证明是一种可靠的鉴定中华蟾蜍复合体中所有 3 个物种的有前途的工具。使用二维图谱作为指纹可以很容易地区分物种。此外，该方法可用于研究杂种形成和半克隆遗传信息转移的机制，当需要快速可靠地鉴定参与该过程的物种时。
