Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
Arch Biochem Biophys. 2012 Apr 15;520(2):67-73. doi: 10.1016/j.abb.2012.02.012. Epub 2012 Mar 3.
Amifostine is the only approved radioprotective agent by FDA for reducing the damaging effects of radiation on healthy tissues. In this study, the protective effect of amifostine against the damaging effects of ionizing radiation on the white matter (WM) and grey matter (GM) regions of the rat brain were investigated at molecular level. Sprague-Dawley rats, which were administered amifostine or not, were whole-body irradiated at a single dose of 800 cGy, decapitated after 24 h and the brain tissues of these rats were analyzed using Fourier transform infrared microspectroscopy (FTIRM). The results revealed that the total lipid content and CH(2) groups of lipids decreased significantly and the carbonyl esters, olefinic=CH and CH(3) groups of lipids increased significantly in the WM and GM after exposure to ionizing radiation, which could be interpreted as a result of lipid peroxidation. These changes were more prominent in the WM of the brain. The administration of amifostine before ionizing radiation inhibited the radiation-induced lipid peroxidation in the brain. In addition, this study indicated that FTIRM provides a novel approach for monitoring ionizing radiation induced-lipid peroxidation and obtaining different molecular ratio images can be used as biomarkers to detect lipid peroxidation in biological systems.
氨磷汀是唯一经美国食品和药物管理局批准的用于减少辐射对健康组织损害的放射防护剂。在这项研究中，从分子水平上研究了氨磷汀对电离辐射损伤大鼠脑白质（WM）和灰质（GM）的保护作用。给予氨磷汀或不给予氨磷汀的斯普拉格-道利大鼠全身照射 800cGy 单次剂量，24 小时后断头，用傅里叶变换红外显微镜（FTIRM）分析这些大鼠的脑组织。结果表明，暴露于电离辐射后，WM 和 GM 中的总脂质含量和 CH2 脂质基团显著减少，而羰基酯、烯键=CH 和 CH3 脂质基团显著增加，这可以解释为脂质过氧化的结果。这些变化在脑 WM 中更为明显。电离辐射前给予氨磷汀可抑制脑内辐射诱导的脂质过氧化。此外，本研究表明，FTIRM 为监测电离辐射诱导的脂质过氧化提供了一种新方法，获得不同的分子比图像可以用作生物系统中脂质过氧化的生物标志物。
