Van Beek J H, Bouma P, Westerhof N
Laboratory for Physiology, Free University, Amsterdam, The Netherlands.
Am J Physiol. 1990 Nov;259(5 Pt 2):H1606-8. doi: 10.1152/ajpheart.1990.259.5.H1606.
In isolated hearts perfused with salt solutions, albumin is often added in low concentrations for preservation of endothelial function. Albumin in low concentrations is also sometimes used as an intravascular indicator in such preparations. We perfused isolated rabbit hearts according to Langendorff with a constant flow of Tyrode solution. Despite maximal vasodilation with 10 microM adenosine, coronary vascular resistance increased by 14% after 0.3 g/100 ml nondefatted bovine serum albumin (Sigma A-9647) was added to the perfusate compared with the control resistance with no albumin present. The 50% response time for the vasoconstrictive response to the step change in albumin concentration was 20 +/- 13 s (mean +/- SD). When 0.3 g/100 ml of defatted albumin (Sigma A-6003) was added, the resistance increase was only 1.4% and is explained by increased viscosity. Vasoconstrictive effects of certain nondefatted albumin preparations must be taken into account by investigators who perfuse organs with albumin-containing solutions.
在灌注盐溶液的离体心脏中，常添加低浓度白蛋白以维持内皮功能。低浓度白蛋白有时也用作此类制剂中的血管内指示剂。我们按照Langendorff法用恒流的台氏液灌注离体兔心。尽管用10微摩尔腺苷实现了最大程度的血管舒张，但与无白蛋白的对照阻力相比，在灌注液中添加0.3克/100毫升未脱脂牛血清白蛋白（Sigma A - 9647）后，冠状动脉血管阻力增加了14%。白蛋白浓度阶跃变化引起的血管收缩反应的50%反应时间为20±13秒（平均值±标准差）。当添加0.3克/100毫升脱脂白蛋白（Sigma A - 6003）时，阻力增加仅为1.4%，这可由粘度增加来解释。用含白蛋白溶液灌注器官的研究人员必须考虑某些未脱脂白蛋白制剂的血管收缩作用。
