National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.
Photochem Photobiol Sci. 2012 Jul;11(7):1156-63. doi: 10.1039/c2pp05411f. Epub 2012 Mar 9.
Photodynamic therapy (PDT) has been the subject of several clinical studies. Evidence to date suggests that direct cell death may involve apoptosis. T(24) cells (bladder cancer cells, ATCC-Nr. HTB-4) were subjected to PDT with aluminum phthalocyanine tetrasulfonate chloride (AlS(4)Pc-Cl) and red laser light at 670 nm. Morphological changes after PDT were visualized under confocal microscopy. Raman microspectroscopy is considered as one of the newly established methods used for the detection of cytochrome c as an apoptotic marker. Results showed that PDT treated T(24) cells seem to undergo apoptosis after irradiation with 3 J cm(-2). Cytochrome c could not be detected from cells incubated with AlS(4)Pc-Cl using Raman spectroscopy whereas AlS(4)Pc-Cl seems to interfere with the Raman spectrum of cytochrome c.
光动力疗法（PDT）已经成为了多项临床研究的课题。目前的证据表明，直接的细胞死亡可能涉及细胞凋亡。将 T（24）细胞（膀胱癌细胞，ATCC-Nr. HTB-4）用铝酞菁四磺酸钠氯（AlS（4）Pc-Cl）和 670nm 的红色激光进行 PDT。在共聚焦显微镜下观察 PDT 后的形态变化。拉曼微光谱被认为是一种新建立的方法，用于检测细胞色素 c 作为凋亡标志物。结果表明，用 3Jcm（-2）照射后，PDT 处理的 T（24）细胞似乎经历了细胞凋亡。用拉曼光谱法从用 AlS（4）Pc-Cl 孵育的细胞中无法检测到细胞色素 c，而 AlS（4）Pc-Cl 似乎干扰了细胞色素 c 的拉曼光谱。
