Landgraf R, Malkinson T J, Veale W L, Lederis K, Pittman Q J
Department of Cell Biology and Regulation, University of Leipzig, Germany.
Am J Physiol. 1990 Nov;259(5 Pt 2):R1056-62. doi: 10.1152/ajpregu.1990.259.5.R1056.
Urethan-anesthetized rats were used to identify effective stimuli for the release of the peptides arginine vasopressin (AVP) and oxytocin into the ventral septal area (VSA) of the brain. Febrile responses to intracerebroventricular injection of prostaglandin E1 (PGE1) were observed in rats whose body temperatures were maintained at 35, 37, or 39 degrees C. Microinjection of the AVP antagonist d(CH2)5Tyr(Me)AVP into the VSA enhanced fever only when PGE1 administration was associated with a significant rise in body temperature. Passive elevation ("artificial fever") or reduction of body temperature in the absence of a PGE1 stimulus was not affected by the antagonist. Push-pull perfusion of the VSA and the dorsal hippocampus, followed by radioimmunoassay of perfusates for AVP and oxytocin, revealed enhanced release into the VSA of AVP only when PGE1 administration was followed by a rise in body temperature. Oxytocin was released whenever body temperature was raised. Peptide concentrations in simultaneous perfusates of dorsal hippocampus did not change in response to PGE1 administration or to passive elevation of body temperature. We conclude that AVP is released into the VSA, but not the dorsal hippocampus, of the rat during a fever induced by PGE1. Oxytocin is released into the VSA, but not the hippocampus, when temperature is elevated.
使用乌拉坦麻醉的大鼠来确定能促使精氨酸加压素（AVP）和催产素释放到大脑腹侧隔区（VSA）的有效刺激。在体温维持在35、37或39摄氏度的大鼠中观察到对脑室内注射前列腺素E1（PGE1）的发热反应。仅当给予PGE1与体温显著升高相关时，向VSA微量注射AVP拮抗剂d(CH2)5Tyr(Me)AVP才会增强发热。在没有PGE1刺激的情况下被动升高（“人工发热”）或降低体温不受该拮抗剂影响。对VSA和背侧海马进行推挽式灌注，然后对灌注液进行AVP和催产素的放射免疫测定，结果显示仅当给予PGE1后体温升高时，AVP才会增强释放到VSA中。每当体温升高时催产素就会释放。背侧海马同时灌注液中的肽浓度在给予PGE1或被动升高体温后没有变化。我们得出结论，在PGE1诱导的发热期间，AVP释放到大鼠的VSA中，而不是背侧海马中。当体温升高时，催产素释放到VSA中，而不是海马中。
