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关于小球藻中一种酶催化GDP-D-甘露糖可逆差向异构化为GDP-L-半乳糖的机制的观察。

Observations on the mechanism of the reversible epimerization of GDP-D-mannose to GDP-L-galactose by an enzyme from Chlorella pyrenoidosa.

作者信息

Barber G A

出版信息

J Biol Chem. 1979 Aug 25;254(16):7600-3.

PMID:224034
Abstract

An enzyme fraction from the green alga Chlorella pyrenoidosa that catalyzes the reversible epimerization of guanosine 5'-diphosphate D-mannose to guanosine 5'-diphosphate L-galactose brings about the incorporation of tritium from tritium-labeled water into the hexosyl moieties of those sugar nucleotides. The hexoses were degraded by periodate oxidation whereby the tritium was found to be equally distributed between carbon atoms 3 and 5. That observation was taken to imply that the epimerizations proceed via ene-diol intermediates.

摘要

来自绿藻蛋白核小球藻的一种酶组分，可催化鸟苷5'-二磷酸-D-甘露糖可逆差向异构化为鸟苷5'-二磷酸-L-半乳糖，该酶能使氚从氚标记的水中掺入到那些糖核苷酸的己糖部分。通过高碘酸盐氧化降解己糖，发现氚在碳原子3和5之间均匀分布。该观察结果表明差向异构化反应是通过烯二醇中间体进行的。

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