从小龙虾外骨骼中提取DNA。

DNA extraction from crayfish exoskeleton.

作者信息

Li Yanhe, Wang Weimin, Liu Xiaolian, Luo Wei, Zhang Jie, Gul Yasmeen

机构信息

College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R.China.

出版信息

Indian J Exp Biol. 2011 Dec;49(12):953-7.

PMID:22403870

Abstract

Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research.

摘要

小龙虾外骨骼（CE）样本通常侵入性较小且易于采集。然而，从它们中提取DNA却很困难。本研究旨在探究将CE作为DNA来源，并设计一种用于聚合酶链反应的简单高效的DNA提取方案。使用特异性引物对（PPO-F，PPO-R）扩增从CE中提取的DNA，并与小龙虾尾部肌肉DNA样本进行比较。此外，使用七个微卫星标记对CE DNA样本集进行扩增。由于从CE中提取的DNA适用于基因扩增，结果表明CE作为基于PCR的群体遗传学研究的一种简单便捷的DNA来源具有实用性。