Hooper Luke, Jenkins Tom L, Griffiths Andrew M, Moore Karen A, Stevens Jamie R
Department of Biosciences, College of Life and Environmental Sciences, Hatherly Laboratories, University of Exeter, Exeter, UK.
Mitochondrial DNA B Resour. 2021 Oct 23;6(11):3309-3311. doi: 10.1080/23802359.2021.1994899. eCollection 2021.
The pink sea fan, (Pallas, 1766), inhabits rocky substrates across the northeast Atlantic and the western Mediterranean. Across much of its range it has been detrimentally affected by fishing. DNA from 17 specimens was amplified by phi29-induced rolling circle amplification. Following purification by sodium acetate-ethanol precipitation, the circular genomic DNA was sequenced on an Illumina MiSeq v2. Specimens originated from sites along the west coast of Ireland, southwest Wales, southwest/southern England, northwest France, southern Portugal, and the Mediterranean coast of northeast Spain. All samples had identical mitochondrial genome sequences of 19,267 bp and included 14 protein-coding genes (including the gene), two ribosomal RNA subunits ( and ) and one methionine tRNA gene. Two genes ( and ) overlapped by 13 bp; all other genes were separated by non-coding intergenic regions. All protein-coding genes had the same start codon (ATG) and a TAA or TAG stop codon, except for that terminated with the incomplete stop codon T--. The mitochondrial genome of (MW588805) showed 99.72% similarity with that of a related sea fan species, , with six SNPs and a 49 bp deletion between and in distinguishing the two.
粉红海扇（Pallas，1766年）栖息于东北大西洋和地中海西部的岩石基质上。在其分布的大部分区域，它受到捕捞的不利影响。通过phi29诱导的滚环扩增对17个样本的DNA进行了扩增。经醋酸钠-乙醇沉淀纯化后，在Illumina MiSeq v2上对环状基因组DNA进行了测序。样本来自爱尔兰西海岸、威尔士西南部、英格兰西南部/南部、法国西北部、葡萄牙南部以及西班牙东北部地中海沿岸的地点。所有样本的线粒体基因组序列均为19267 bp，包含14个蛋白质编码基因（包括 基因）、两个核糖体RNA亚基（ 和 ）和一个甲硫氨酸tRNA基因。两个基因（ 和 ）重叠13 bp；所有其他基因被非编码基因间隔区隔开。除了 以不完整的终止密码子T--结束外，所有蛋白质编码基因都具有相同的起始密码子（ATG）和TAA或TAG终止密码子。粉红海扇（MW588805）的线粒体基因组与相关海扇物种 的线粒体基因组显示出99.72% 的相似性，在 和 之间有六个单核苷酸多态性（SNP）和一个49 bp的缺失来区分这两个物种。
