Li Zhe, Yan Zhiyong, Chen Shiyan, Wang Huaping
College of Materials Science and Engineering, Donghua University, State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai 201620, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2012 Feb;29(1):164-9.
Bacterial cellulose (BC) was prepared by Acetobacter xylinum in static culture. After purified by chemical treatment, the microstructure, chemical structure, crystal structure and mechanical property of BC were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD) and tensile strength measurement respectively, and compared with those of the imported bacterial cellulose wound dressing served as control sample (XBC). The results indicated that the diameter of the BC was (22 +/- 9) nm, and the crystallinity index was 89.71%. The tensile strength and the Young's mouduls of BC were significant higher than XBC both in wet and dry states. The biocompatibility of BC and XBC were evaluated by cytotoxicity test, delayed contact sensitization study in the Guinea Pig and skin irritation test. The results showed that BC had reliable biocompatibility as well as XBC. With the unique nanostructure, high crystallinity, high mechanical strength, and reliable biocompatibility, BC produced in our country as well as XBC can be used as a safe biomaterial for the medical applications.
木醋杆菌在静态培养条件下制备细菌纤维素（BC）。经化学处理纯化后，分别采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、X射线衍射仪（XRD）和拉伸强度测量对BC的微观结构、化学结构、晶体结构和力学性能进行表征，并与作为对照样品的进口细菌纤维素伤口敷料（XBC）进行比较。结果表明，BC的直径为（22±9）nm，结晶度指数为89.71%。BC在湿态和干态下的拉伸强度和杨氏模量均显著高于XBC。通过细胞毒性试验、豚鼠迟发型接触致敏研究和皮肤刺激性试验对BC和XBC的生物相容性进行评价。结果表明，BC与XBC一样具有可靠的生物相容性。我国生产的BC与XBC具有独特的纳米结构、高结晶度、高机械强度和可靠的生物相容性，可作为安全的生物材料用于医学应用。
