Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology, Mol, Belgium.
Bioresour Technol. 2012 May;111:368-77. doi: 10.1016/j.biortech.2012.02.043. Epub 2012 Feb 20.
A continuous cultivation of Clostridium acetobutylicum ATCC 824 is described using a two-stage design to mimic the two phases of batch culture growth of the organism. A hydrophobic pervaporation unit was coupled to the second fermentor containing the highest solvent titers. This in situ product recovery technology efficiently decreased butanol toxicity in the fermentor while the permeate was enriched to 57-195 g L(-1) total solvents depending on the solvent concentrations in the fermentor. By the alleviation of product inhibition, the glucose concentration could be increased from 60 to 126 g L(-1) while the productivity increased concomitantly from 0.13 to 0.30 g L(-1)h(-1). The continuous fermentation was conducted for 1172 h during which the pervaporation was coupled to the second fermentor for 475 h with an average flux of 367 g m(-2)h(-1). The energy consumption was calculated for a 2 wt.% n-butanol fermentation broth and compared with the conventional process.
连续培养丙酮丁醇梭菌 ATCC824 采用两阶段设计，模拟该生物体分批培养的两个阶段。将疏水性渗透蒸发装置与含有最高溶剂浓度的第二发酵罐耦合。该原位产物回收技术有效地降低了发酵罐中丁醇的毒性，同时渗透物中总溶剂浓度富集到 57-195 g/L，具体取决于发酵罐中的溶剂浓度。通过缓解产物抑制，葡萄糖浓度可以从 60 增加到 126 g/L，同时生产力从 0.13 增加到 0.30 g/L·h-1。连续发酵进行了 1172 h，在此期间，渗透蒸发与第二发酵罐耦合了 475 h，平均通量为 367 g·m-2·h-1。计算了 2 wt.%正丁醇发酵液的能量消耗，并与传统工艺进行了比较。
