Division of Infectious Diseases, Carol Yu Centre for Infection and Department of Microbiology, The University of Hong Kong, Queen Mary hospital, Hong Kong Special Administrative Region, PR China.
J Infect. 2012 May;64(5):500-6. doi: 10.1016/j.jinf.2012.02.018. Epub 2012 Mar 7.
To investigate the prevalence and molecular epidemiology of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) nasal carriage in children.
We collected nasal and nasopharyngeal swabs from 2211 children aged 2-5 years attending 79 day care centers (DCCs) and 113 kindergartens (KGs) in all 18 geographical districts in Hong Kong.
The overall carriage rates of S. aureus and MRSA were 27.6% (95% confidence interval [CI], 24.8-28.5%) and 1.3% (95% CI, 0.8-1.8%), respectively. Molecular typing (staphylococcal cassette chromosome mec [SCCmec], sequence type [ST], clonal cluster [CC]) showed that all the 28 MRSA isolates had SCCmec IV (n = 13) or V (n = 15) including 12 isolates with community-associated-MRSA genotypes (ST59-IV/V, ST30-IV and ST88-V), 10 isolates with healthcare-associated-MRSA genotypes (ST45-IV/V, CC5-IV and ST630-V) and six isolates with novel genotypes (ST10-V and CC1-IV). Spa typing indicated that there was some within and between DCCs/KGs transmission of certain MRSA and methicillin-sensitive S. aureus strains but this was not extensive.
Our findings indicate the potential for DCCs to be a reservoir for emerging MRSA genotypes and highlight the need to enhance education and infection control measures to reduce their cross-transmission in this population.
调查儿童鼻腔金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）定植的流行率和分子流行病学特征。
我们收集了 2211 名 2-5 岁儿童的鼻腔和鼻咽拭子，这些儿童来自香港所有 18 个地理区域的 79 个日托中心（DCC）和 113 个幼儿园（KG）。
金黄色葡萄球菌和 MRSA 的总定植率分别为 27.6%（95%置信区间[CI]，24.8-28.5%）和 1.3%（95% CI，0.8-1.8%）。分子分型（葡萄球菌盒染色体 mec [SCCmec]、序列型[ST]、克隆群[CC]）显示，28 株 MRSA 分离株均携带 SCCmec IV（n = 13）或 V（n = 15），包括 12 株具有社区相关-MRSA 基因型（ST59-IV/V、ST30-IV 和 ST88-V）、10 株具有医疗保健相关-MRSA 基因型（ST45-IV/V、CC5-IV 和 ST630-V）和 6 株具有新型基因型（ST10-V 和 CC1-IV）。Spa 分型表明，某些 MRSA 和甲氧西林敏感金黄色葡萄球菌菌株在 DCC/KG 之间存在一定的内部和外部传播，但这种传播并不广泛。
我们的研究结果表明，DCC 可能成为新兴 MRSA 基因型的储库，并强调需要加强教育和感染控制措施，以减少这些人群之间的交叉传播。
