Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
J Chromatogr A. 2012 Apr 13;1233:152-5. doi: 10.1016/j.chroma.2012.01.074. Epub 2012 Feb 1.
Externally generated pH gradients are employed on a multimodal cation exchange chromatographic resin to improve the selectivity for a mixture of model proteins. By combining controlled pH gradients with the unique selectivities arising from the multiple interaction types exhibited by the multimodal resin, the separation of the protein mixture is significantly improved as compared to linear salt gradient operation. Several gradient conditions are explored and a shallow gradient from pH 3.8 to 5.5 is shown to be able to resolve the proteins. This work provides proof of concept for the use of pH gradients in multimodal chromatography and sets the stage for future applications.
在外源性 pH 梯度的作用下，多模式阳离子交换树脂被用于改善模型蛋白混合物的选择性。通过将 pH 梯度控制与多模式树脂所表现出的多种相互作用类型的独特选择性相结合，与线性盐梯度操作相比，蛋白质混合物的分离得到了显著改善。本文探索了几种梯度条件，并表明 pH 从 3.8 到 5.5 的浅梯度能够实现蛋白质的分离。这项工作为多模式色谱中 pH 梯度的应用提供了概念验证，并为未来的应用奠定了基础。
