Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram, Kerala, India.
Biomed Mater. 2012 Jun;7(3):035005. doi: 10.1088/1748-6041/7/3/035005. Epub 2012 Mar 9.
This study was undertaken to glean preliminary information on the role of triphasic ceramic coated hydroxyapatite (HASi) and biphasic (alpha-tricalcium phosphate and hydroxyapatite based) calcium phosphate (BCP) for the development of osteochondral constructs. The proposed constructs were tested for performance in vitro with rabbit adipose-derived mesenchymal stem cells (RADMSCs) and further analysed in vivo in a lapine model for osteochondral defect amelioration. Desirable scaffolding architecture ensuring favourable conditions for cell attachment, nutrient exchange and neo-tissue organization was achieved by the synthesis of porous ceramic blocks and characterizations were carried out using x-ray diffraction and Fourier transform infrared spectroscopy. The cytocompatibility of the scaffold-cell combination product was evaluated using microscopy techniques that proved the scaffold to be non-cytotoxic and favourable for cell growth and proliferation. Short-term implantation studies were conducted with bare cylindrical HASi and BCP scaffolds, press fit deep into the bony bed of the median femoral condyles of the rabbit, which resulted in favourable specific in vivo response of de novo cartilage-like cells on the surface and sub-surface bony trabeculae. The generated pilot data will help to assess the severity of proposed procedures before embarking on scaled-up efforts.
本研究旨在初步探讨三相陶瓷涂层羟基磷灰石（HASi）和双相（基于α-磷酸三钙和羟基磷灰石的磷酸钙）钙磷（BCP）在骨软骨构建中的作用。所提出的构建体通过兔脂肪间充质干细胞（RADMSCs）进行了体外性能测试，并在兔骨软骨缺损改善模型中进行了体内进一步分析。通过多孔陶瓷块的合成实现了理想的支架结构，确保了细胞附着、营养交换和新组织形成的有利条件，并通过 X 射线衍射和傅里叶变换红外光谱进行了表征。使用显微镜技术评估了支架-细胞组合产物的细胞相容性，证明支架无细胞毒性，有利于细胞生长和增殖。将裸柱状 HASi 和 BCP 支架短时间植入兔股骨髁中部骨床深处，结果表明表面和亚表面骨小梁上有新形成的类软骨细胞的有利特定体内反应。所生成的初步数据将有助于在进行规模化努力之前评估拟议程序的严重程度。
