Instituto de Biología Molecular y Celular de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
Chembiochem. 2012 Mar 19;13(5):732-9. doi: 10.1002/cbic.201100678. Epub 2012 Mar 8.
Selective isotopic unlabeling of proteins can provide important residue-type information as well as reduce congestion of NMR spectra. However, metabolic scrambling often complicates the final isotope-labeling pattern. Here, an array of metabolic precursors is used to perform robust, residue-specific unlabeling of proteins. The resulting isotopic-labeling patterns are predictable and nicely complement NMR experiments that differentiate residue types. This approach has widespread applications, but it is particularly relevant for proteins that lack sequence complexity or a defined tertiary structure.
选择性同位素标记蛋白质可以提供重要的残基类型信息，同时减少 NMR 光谱的拥挤。然而，代谢重排常常使最终的同位素标记模式复杂化。在这里，使用一组代谢前体来进行稳健的、残基特异性的蛋白质去标记。得到的同位素标记模式是可预测的，并且很好地补充了区分残基类型的 NMR 实验。这种方法有广泛的应用，但对于缺乏序列复杂性或定义明确的三级结构的蛋白质尤其相关。
