Sugiki Toshihiko, Kobayashi Naohiro, Fujiwara Toshimichi
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Comput Struct Biotechnol J. 2017 Apr 13;15:328-339. doi: 10.1016/j.csbj.2017.04.001. eCollection 2017.
Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for structural studies of chemical compounds and biomolecules such as DNA and proteins. Since the NMR signal sensitively reflects the chemical environment and the dynamics of a nuclear spin, NMR experiments provide a wealth of structural and dynamic information about the molecule of interest at atomic resolution. In general, structural biology studies using NMR spectroscopy still require a reasonable understanding of the theory behind the technique and experience on how to recorded NMR data. Owing to the remarkable progress in the past decade, we can easily access suitable and popular analytical resources for NMR structure determination of proteins with high accuracy. Here, we describe the practical aspects, workflow and key points of modern NMR techniques used for solution structure determination of proteins. This review should aid NMR specialists aiming to develop new methods that accelerate the structure determination process, and open avenues for non-specialist and life scientists interested in using NMR spectroscopy to solve protein structures.
核磁共振(NMR)光谱学是一种用于研究化合物和生物分子(如DNA和蛋白质)结构的强大技术。由于NMR信号能灵敏地反映核自旋的化学环境和动力学,NMR实验能在原子分辨率下提供有关目标分子的丰富结构和动力学信息。一般来说,使用NMR光谱学进行结构生物学研究仍需要对该技术背后的理论有合理的理解,以及掌握如何记录NMR数据的经验。由于过去十年的显著进展,我们可以轻松获取合适且常用的分析资源,用于高精度测定蛋白质的NMR结构。在此,我们描述用于蛋白质溶液结构测定的现代NMR技术的实际操作、工作流程和关键点。这篇综述应有助于旨在开发加速结构测定过程新方法的NMR专家,并为有兴趣使用NMR光谱学解决蛋白质结构问题的非专业人士和生命科学家开辟道路。