Pei Yan-zhen, Han Tao, Ma Xiao-yan, Li Ying, Xing Jing, Song Zuo-li
Department of Hepatology, Tianjin Institute of Hepatobiliary Disease, Tianjin, China.
Zhonghua Gan Zang Bing Za Zhi. 2011 Oct;19(10):743-6. doi: 10.3760/cma.j.issn.1007-3418.2011.10.006.
To investigate the discrepancy of HBsAg titre and correlation of HBV DNA levels among patients with chronic hepatitis B (CHB), HBV-related liver cirrhosis (LC) and hepatocellular carcinoma (HCC).
HBsAg titre and HBV DNA in serum samples were measured among 47 CHB, 72 LC and 54 HCC cases using Abbott chemiluminescence and fluorescence quantitative PCR, respectively. Statistical analyses among multiple groups, between two groups and about the correlation were performed using Kruskal-Wallis test, Mann-Whitney U test and Spearman test, respectively.
The median HBsAg titre level in serum samples decreased from 2361.10 IU/ml in CHB cohort to 1001.64 IU/ml in LC cohort and 594.35 IU/ml in HCC cohort, suggesting a statistically significant difference (x2 = 24.394, P less than 0.05). Moreover, HBsAg titre in CHB group was significantly higher than that in LC group ( Z = -3.754, P less than 0.05). CHB patients had significantly higher HBsAg titre than HCC cases ( Z = -4.630, P less than 0.05). However, there was no statistically significant difference in HBsAg titre between LC and HCC group. Among HBeAg positive patients, HBsAg titre decreased from 3259.83 IU/ml in CHB group to 1077.30 IU/ml in LC group and 789.72 IU/ml in HCC group, indicating a significant difference (x2 = 15.643, P less than 0.01). Among HBeAg negative patients, HBsAg titre declined from 1669.00 IU/ml in CHB group to 1001.64 IU/ml in LC group and 582.05 IU/ml in HCC group, suggesting of a significant difference (x2 = 6.423, P less than 0.05). Positive correlation between HBsAg titre and HBV DNA was found in CHB ( r = 0.297, P less than 0.05), LC (r = 0.346, P less than 0.05) and HCC (r = 0.452, P less than 0.05), respectively.
HBsAg titre level in serum decreased progressively from CHB to LC and HCC group. There were positive correlations between HBsAg titre and HBV DNA level in CHB, LC and HCC.
探讨慢性乙型肝炎（CHB）、HBV相关肝硬化（LC）和肝细胞癌（HCC）患者的HBsAg滴度差异及HBV DNA水平的相关性。
分别采用雅培化学发光法和荧光定量PCR法检测47例CHB、72例LC和54例HCC患者血清样本中的HBsAg滴度和HBV DNA。多组间、两组间及相关性的统计分析分别采用Kruskal-Wallis检验、Mann-Whitney U检验和Spearman检验。
血清样本中HBsAg滴度中位数从CHB队列中的2361.10 IU/ml降至LC队列中的1001.64 IU/ml和HCC队列中的594.35 IU/ml，差异有统计学意义（x2 = 24.394，P＜0.05）。此外，CHB组的HBsAg滴度显著高于LC组（Z = -3.754，P＜0.05）。CHB患者的HBsAg滴度显著高于HCC患者（Z = -4.630，P＜0.05）。然而，LC组和HCC组之间的HBsAg滴度差异无统计学意义。在HBeAg阳性患者中，HBsAg滴度从CHB组的3259.83 IU/ml降至LC组的1077.30 IU/ml和HCC组的789.72 IU/ml，差异有统计学意义（x2 = 15.643，P＜0.01）。在HBeAg阴性患者中，HBsAg滴度从CHB组的1669.00 IU/ml降至LC组的1001.64 IU/ml和HCC组的582.05 IU/ml，差异有统计学意义（x2 = 6.423，P＜0.05）。CHB（r = 0.297，P＜0.05）、LC（r = 0.346，P＜0.05）和HCC（r = 0.452，P＜0.05）患者的HBsAg滴度与HBV DNA之间均呈正相关。
血清中HBsAg滴度水平从CHB组到LC组和HCC组逐渐降低。CHB、LC和HCC患者的HBsAg滴度与HBV DNA水平之间均呈正相关。
