Liu Xing-hui, Zhou Xin, Zhu Cheng-liang, Song Hui, Liu Fang
Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan,China.
Zhonghua Gan Zang Bing Za Zhi. 2011 Oct;19(10):751-4. doi: 10.3760/cma.j.issn.1007-3418.2011.10.008.
To investigate the effect of core protein of hepatitis C virus (HCV) on the expression of hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF).
Huh7.5.1 cells were transfected with plasmid flag2B-core carrying HCV core gene, expression of HIF-1alpha and VEGF were measured by reverse transcription-polymorphism chain reaction (RT-PCR) and western blot. Enzyme link immunoabsorbent assay (ELISA) were used to detect the level of VEGF in the supernatants.
The expression of HIF-1alpha and VEGF mRNA and protein were upregulated after flag2B-core was transfected into Huh7.5.1 cells, and VEGF level in the supernatant was significant elevated as compared to controls [(654.5+/-43.7) pg/ml vs (365.9+/-26.8) pg/ml, t = 653.1%, P less than 0.01]. The expression of HIF-1alpha and VEGF mRNA and protein were downregulated after flag2B-core and HIF-1alpha siRNA were co-transfected into Huh7.5.1 cells, and VEGF level in the supernatant was significantly reduced as compared to controls [(389.2+/-29.6) pg/ml vs (768.8+/-47.3)pg/ml, t = 1330.22, P less than 0.01].
HCV core protein enhances the expression of HIF-1alpha and VEGF. HCV may regulate the expression of HIF-1alpha and VEGF via the core protein.
研究丙型肝炎病毒（HCV）核心蛋白对缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）表达的影响。
用携带HCV核心基因的质粒flag2B-core转染Huh7.5.1细胞，采用逆转录-多态性链反应（RT-PCR）和蛋白质免疫印迹法检测HIF-1α和VEGF的表达。用酶联免疫吸附测定法（ELISA）检测上清液中VEGF的水平。
将flag2B-core转染至Huh7.5.1细胞后，HIF-1α和VEGF mRNA及蛋白表达上调，与对照组相比，上清液中VEGF水平显著升高[（654.5±43.7）pg/ml对（365.9±26.8）pg/ml，t = 653.1%，P<0.01]。将flag2B-core与HIF-1α siRNA共转染至Huh7.5.1细胞后，HIF-1α和VEGF mRNA及蛋白表达下调，与对照组相比，上清液中VEGF水平显著降低[（389.2±29.6）pg/ml对（768.8±47.3）pg/ml，t = 1330.22，P<0.01]。
HCV核心蛋白增强HIF-1α和VEGF的表达。HCV可能通过核心蛋白调节HIF-1α和VEGF的表达。
