Boxberger H J, Paweletz N
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.
Anticancer Res. 1990 Sep-Oct;10(5A):1265-73.
In a previous paper we studied the influences of the basal lamina and its components on the invasive but nonmetastatic variant BSp 73 AS of a rat pancreatic adenocarcinoma. In continuation of this publication we used the in vivo strongly metastasizing but hardly invasive variant BSp 73 ASML. On plastic or glass supports the low passaged ASML-cells grew separately, spread very scarcely and remained passive. Compared to these standard culture conditions, the behaviour of the tumour cells could be modified by biomaterials like collagen type I/III and type IV, fibronectin, laminin, matrigel, lens capsule and basal lamina. On these substrata (compared to plastic supports) the ASML-cells showed increased spreading, filopodial outgrowth and secretion of membrane vesicles. Only signs of motility as well as penetration of the biomatrices could be observed. Interestingly, highly passaged ASML-cells displayed an increased tendency to spread even on plastic. Under dynamic conditions these cells even failed to adhere. Obviously the biomatrices in vitro as well as long-term passaging are able to lead the ASML-cells partly to reactivate the behaviour that is characteristic of malignant tumour cells. Additionally, there is some evidence that the ASML-cells in vitro can strongly respond to chemotactic stimuli by exhibiting both flattening and translocative motility.
在之前的一篇论文中，我们研究了基底膜及其成分对大鼠胰腺腺癌侵袭性但非转移性变体BSp 73 AS的影响。作为该出版物的延续，我们使用了体内强烈转移但几乎无侵袭性的变体BSp 73 ASML。在塑料或玻璃支持物上，低传代的ASML细胞单独生长，很少伸展，保持静止状态。与这些标准培养条件相比，肿瘤细胞的行为可以被诸如I/III型和IV型胶原、纤连蛋白、层粘连蛋白、基质胶、晶状体囊膜和基底膜等生物材料所改变。在这些基质上（与塑料支持物相比），ASML细胞表现出伸展增加、丝状伪足生长和膜泡分泌。仅观察到运动迹象以及生物基质的穿透。有趣的是，高传代的ASML细胞即使在塑料上也表现出增加的伸展倾向。在动态条件下，这些细胞甚至无法黏附。显然，体外的生物基质以及长期传代能够使ASML细胞部分地重新激活恶性肿瘤细胞特有的行为。此外，有一些证据表明，体外的ASML细胞通过表现出扁平化和易位运动，能够对趋化刺激产生强烈反应。
