National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
PLoS One. 2012;7(3):e32860. doi: 10.1371/journal.pone.0032860. Epub 2012 Mar 6.
The brown planthopper (BPH), Nilaparvata lugens (Stå;l), which belongs to Homopteran, Delphacidae, is one of the most serious and destructive pests of rice. Feeding BPH with homologous dsRNA in vitro can lead to the death of BPH, which gives a valuable clue to the prevention and control of this pest, however, we know little about its small RNA world.
METHODOLOGY/PRINCIPAL FINDINGS: Small RNA libraries for three developmental stages of BPH (CX-male adult, CC-female adult, CY-last instar female nymph) had been constructed and sequenced. It revealed a prolific small RNA world of BPH. We obtained a final list of 452 (CX), 430 (CC), and 381 (CY) conserved microRNAs (miRNAs), respectively, as well as a total of 71 new miRNAs in the three libraries. All the miRNAs had their own expression profiles in the three libraries. The phylogenic evolution of the miRNA families in BPH was consistent with other species. The new miRNA sequences demonstrated some base biases.
Our study discovered a large number of small RNAs through deep sequencing of three small RNA libraries of BPH. Many animal-conserved miRNA families as well as some novel miRNAs have been detected in our libraries. This is the first achievement to discover the small RNA world of BPH. A lot of new valuable information about BPH small RNAs has been revealed which was helpful for studying insect molecular biology and insect resistant research.
褐飞虱（BPH），Nilaparvata lugens（Stål），属于同翅目，飞虱科，是水稻最严重和最具破坏性的害虫之一。在体外用同源 dsRNA 喂养 BPH 会导致 BPH 死亡，这为这种害虫的防治提供了宝贵的线索，然而，我们对其小 RNA 世界知之甚少。
方法/主要发现：已经构建和测序了三个 BPH 发育阶段（CX-雄性成虫、CC-雌性成虫、CY-最后龄期雌性若虫）的小 RNA 文库。它揭示了 BPH 丰富的小 RNA 世界。我们分别获得了 452（CX）、430（CC）和 381（CY）个保守 microRNAs（miRNAs）的最终列表，以及三个文库中总共 71 个新 miRNAs。所有 miRNAs 在这三个文库中都有自己的表达谱。miRNA 家族在 BPH 中的系统发育进化与其他物种一致。新 miRNA 序列显示出一些碱基偏好。
我们通过对三个 BPH 小 RNA 文库的深度测序发现了大量小 RNA。在我们的文库中检测到了许多动物保守的 miRNA 家族和一些新的 miRNAs。这是首次发现 BPH 的小 RNA 世界。揭示了大量关于 BPH 小 RNA 的新有价值信息，有助于研究昆虫分子生物学和昆虫抗性研究。
