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用于点扫描显微镜的椭圆镜聚焦的严格理论。

Rigorous theory on elliptical mirror focusing for point scanning microscopy.

作者信息

Liu Jian, Tan Jiubin, Wilson Tony, Zhong Cien

机构信息

Ultra-Precision Optoelectronic Instrument Engineering Center, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin 150001, Heilongjiang, China.

出版信息

Opt Express. 2012 Mar 12;20(6):6175-84. doi: 10.1364/OE.20.006175.

Abstract

A rigorous elliptical mirror focusing formula based on spherical wave transformation is derived as a kind of imaging technique with high NA for potential applications in molecule imaging, spectroscopy and industrial artifact microscopy. An apodization factor is given and used to compare the energy conversation rules in lens transmission and parabolic and elliptical mirror reflections. Simulation results indicate that the axial HFWHM of elliptical and parabolic mirrors is about 80% of the corresponding HFWHM of lens in case of NA = 1 and φs = 0, and the side lobe noise is also slightly lower than that of lens, but the transverse HFWHM of mirrors is comparatively wider despite the width of main lobe is still smaller. In comparison with parabolic mirror based system, an elliptical mirror based system is potentially promising in aberration control of incident beam when the aperture of mirror is enlarged to adapt a large stage or specimen container at a small beam shading ratio.

摘要

基于球面波变换推导了一种严格的椭圆镜聚焦公式,作为一种高数值孔径成像技术,有望应用于分子成像、光谱学和工业工件显微镜检查。给出了一个变迹因子,并用于比较透镜透射以及抛物面镜和椭圆面镜反射中的能量守恒规则。模拟结果表明,在数值孔径NA = 1且φs = 0的情况下,椭圆镜和抛物面镜的轴向半高宽约为透镜相应半高宽的80%,旁瓣噪声也略低于透镜,但尽管主瓣宽度仍然较小,镜的横向半高宽相对较宽。与基于抛物面镜的系统相比,当镜的孔径扩大以适应大载物台或标本容器且光束遮挡率较小时,基于椭圆镜的系统在控制入射光束像差方面具有潜在的优势。

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