Iwamuro Yoshiaki, Iio-Ishimaru Reiko, Chinaka Satoshi, Takayama Nariaki, Hayakawa Kazuichi
Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters, 1-1 Kuratsuki, Kanazawa, 920-8553, Japan.
Biomed Chromatogr. 2012 Nov;26(11):1452-6. doi: 10.1002/bmc.2719. Epub 2012 Mar 14.
Δ(9) -Tetrahydrocannabinol is the primary psychoactive component in cannabis, one of the most commonly used illicit drugs in the world. This paper describes a simple and rapid method for direct analysis of major metabolites of Δ(9) -tetrahydrocannabinol; 11-nor-Δ(9) -tetrahydrocannabinol-9-carboxylic acid and its glucuronide in urine by capillary electrophoresis/mass spectrometry. The only pretreatment needed for a urine sample was dilution with methanol containing an internal standard and centrifugation. Electrophoresis was carried out in an untreated fused-silica capillary (50 µm i.d. × 85 cm) filled with 40 m m ammonium formate (pH 6.4). An analysis could be completed within 10 min. For both compounds, the assay was linear over the range 0.1-10 µg/mL in urine with correlation coefficients (r(2) ) >0.99 and the limit of detection was 0.5 pg (10 nL injection). The detection yields and reproducibilities were determined at three different concentrations (0.1, 0.5 and 2 µg/mL in urine). The mean detection yields were 60-99%. The intra- and inter-day relative standard deviations of migration times were 0.063-0.19 and 0.18-0.36%, and those of peak areas were 4.2-18 and 5.9-25%, respectively. The proposed method successfully analyzed the urine samples of cannabis users.
Δ(9)-四氢大麻酚是大麻中的主要精神活性成分，大麻是世界上最常用的非法药物之一。本文描述了一种通过毛细管电泳/质谱直接分析Δ(9)-四氢大麻酚主要代谢物；11-去甲-Δ(9)-四氢大麻酚-9-羧酸及其葡糖醛酸苷在尿液中的简单快速方法。尿液样本唯一需要的预处理是用含有内标的甲醇稀释并离心。电泳在未处理的熔融石英毛细管（内径50 µm×85 cm）中进行，填充40 mM甲酸铵（pH 6.4）。一次分析可在10分钟内完成。对于这两种化合物，该测定在尿液中0.1-10 µg/mL范围内呈线性，相关系数（r(2)）>0.99，检测限为0.5 pg（进样10 nL）。在三种不同浓度（尿液中0.1、0.5和2 µg/mL）下测定检测产率和重现性。平均检测产率为60-99%。迁移时间的日内和日间相对标准偏差分别为0.063-0.19%和0.18-0.36%，峰面积的相对标准偏差分别为4.2-18%和5.9-25%。所提出的方法成功地分析了大麻使用者的尿液样本。
